Mouse myelomas and lymphomas in culture

doi:10.1016/0014-4827(70)90489-1

Experimental Cell Research

Volume 60, Issue 1, April 1970, Pages 61-77

https://doi.org/10.1016/0014-4827(70)90489-1 Get rights and content

Abstract

The establishment and growth characteristics of nine different long-term continuous in vitro cell lines originating from five myeloma anad two lymphoma tumor lines of independent origin in BALB/c or C3H mice, is described. Different culture lines were initiated by somewhat different methods but they now all grow in fortified Eagle's medium supplemented with horse serum as stationary suspension cultures with doubling times between 16 and 26 h. Some of the lines are near tetraploid and contain marker chromosomes while others are diploid or near-diploid.

Several of the myeloma lines synthesize and secrete immunoglobulin while others, probably as a consequence of mutation, do not. The two lymphoma lines may synthesize Ig but do not appear to secrete it.

Assays of the growth inhibiting activities of thymidine and hydrocortisone show that the lymphoma cells are much more sensitive than the myeloma cells. There is evidence suggesting that in these respects, as well as others, the cultured cell lines possess some of the differentiated characteristics of normal lymphocytes and plasma cells. Possible uses of the cell lines to analyze at the biochemical level the modes of genetic control of such characteristics are discussed.

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^☆: This investigation was supported by Public Health Service Research Grant, no. CA-10311-02 from the National Cancer Institute, and Damon Runyon Memorial Fund no. 942, 942A, 942B to Kengo Horibata; a Damon Runyon Memorial Fund Research Fellowship, no. DRF-400 to Alan W. Harris; and by Public Health Service Research Grant, no A105875 from the National Institute of Allergy and Infectious Diseases, and a Public Health Service Training Grant, no. CA0521301 from the National Cancer Institute, both to Dr Melvin Cohn.

¹: Present address: The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital Post Office, Victoria 3050, Australia.

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Mouse myelomas and lymphomas in culture☆

Abstract

Exptl cell res

Immunochemistry

Exptl cell res

Biochem pharmacol

Biochim biophys acta

Biochim biophys acta

Exptl cell res

Exptl cell res

J mol biol

Exptl cell res

J mol biol

J mol biol

J mol biol

Int rev cytol

Biochim biophys acta

Can j genet cytol

Ann N Y acad sci

J natl cancer inst

Ann N Y acad sci