Lack of release of vasoactive intestinal polypeptide and calcitonin gene-related peptide during electrical stimulation of enteric nerves in streptozotocin-diabetic rats

doi:10.1016/0016-5085(87)90567-1

Gastroenterology

Volume 93, Issue 5, November 1987, Pages 1034-1040

https://doi.org/10.1016/0016-5085(87)90567-1 Get rights and content

Abstract

The simultaneous release of endogenous acetylcholine, serotonin, vasoactive intestinal polypeptide, substance P, and calcitonin gene-related peptide was measured during electrical field stimulation of isolated preparations of rat ileum from control and 8-wk streptozotocin-treated diabetic rats. Electrical field stimulation of the control rat ileum caused a significant increase in the release of all the above substances from the enteric nerves. The electrically evoked, but not the basal, release of these substances was inhibited by tetrodotoxin. In the diabetic rat ileum, however, there was no increase in the release of vasoactive intestinal polypeptide and calcitonin gene-related peptide during electrical stimulation, whereas endogenous release of acetylcholine, serotonin, and substance P was unaffected by the diabetic state. This was surprising in view of the increased fluorescence intensity and tissue content of vasoactive intestinal polypeptide-like immunoreactivity in the same tissue reported previously. The lack of increase in evoked release of vasoactive intestinal polypeptide in the diabetic preparations might be due to an impaired mechanism of release at the terminal site or to defective axonal transport of the peptide, whereas in the case of calcitonin gene-related peptide, it might be the result of the low level of the peptide present in the enteric nerve fibers of the diabetic rat ileum. The differential effect of diabetes on enteric nerves is discussed.

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: This work was supported in part by the Medical Research Council.

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Lack of release of vasoactive intestinal polypeptide and calcitonin gene-related peptide during electrical stimulation of enteric nerves in streptozotocin-diabetic rats

Abstract

Lancet

Neuroscience

Neuroscience

Neuroscience

Gastroenterology

Neurosci Lett

Neurosci Lett

Neuroscience

Gastroenterology

Gastroenterology

Gastroenterology

Life Sci

Neuroscieuce

Brain Res

Gastroenterology

Gastroenterology

Regul Pept

Vasoactive intestinal polypeptide: measurement, distribution and putative neurotransmitter function

Digestion

Neuronal peptides in the intestine: distribution and possible functions

Peptide-containing neurones connect the two ganglionated plexuses of the enteric nervous system

Nature

Are there somatostatin containing nerves in the rat gut? Immunohistochemical evidence for a new tvpe of peripheral nerve

Experieentia