Elsevier

Immunochemistry

Volume 6, Issue 1, January 1969, Pages 43-48, IN9-IN11, 49-52
Immunochemistry

Coupling of enzymes to proteins with glutaraldehyde: Use of the conjugates for the detection of antigens and antibodies

https://doi.org/10.1016/0019-2791(69)90177-3Get rights and content

Abstract

Conjugation of peroxidase, glucose oxidase, tyrosinase and alkaline phosphatase to human immunoglobulin-G, human serum albumin, sheep antibody and rabbit antibody was carried out with glutaraldehyde. The conjugates retained a substantial part of their immunological and enzymatic activity. They were utilized for the intracellular detection of antigens and antibodies and they were employed for the characterization of the antibodies after immunoelectrophoresis.

References (25)

  • V. Ghetie

    Immunochemistry

    (1967)
  • S. Avrameas et al.

    Immunochemistry

    (1969)
  • P. Grabar et al.

    Biochim. biophys. Acta

    (1953)
  • A.H. Coons

    Harvey Lecture

    (1958)
  • A.H. Coons et al.

    J. exp. Med.

    (1955)
  • M.C. Berenbaum

    Immunology

    (1959)
  • Y. Yagi et al.

    J. Immun.

    (1962)
  • S. Avrameas et al.

    C.r. Acad. Sci. (Paris)

    (1966)
  • S. Avrameas et al.

    C.r. Acad. Sci. (Paris)

    (1967)
  • P.K. Nakane et al.

    J. Histochem. Cytochem.

    (1966)
  • P.K. Nakane et al.

    J. cell Biol.

    (1967)
  • M. Bouteille et al.

    C.r. Acad. Sci. (Paris)

    (1967)
  • Cited by (0)

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