Species identification of trichostrongyle nematodes by pcr-linked rflp
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2023, Parasitology InternationalShift from morphological to recent advanced molecular approaches for the identification of nematodes
2022, GenomicsCitation Excerpt :Proteolysis, immunology, and the newly created DNA sequencing analysis are examples. Several agriculturally important nematodes have been identified and quantified using restriction fragment length polymorphism (RFLP) [15], random amplified polymorphic DNA (RAPD) [16], isoenzyme pattern analysis [17], polymerase chain reaction (PCR) [18], quantitative polymerase chain reaction (qPCR) [19], amplified fragment length polymorphisms (AFLP) [20], and sequencing of diagnostic rDNA regions [21–23]. Three molecular methods have been used to study free-living nematode biodiversity: Denaturing Gradient Gel Electrophoresis (DGGE), marker gene DNA sequences, and pyrosequencing.
Advances in diagnosis of gastrointestinal nematodes in livestock and companion animals
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2018, Veterinary ParasitologyCitation Excerpt :Four primer/probe sets were constructed: one targeting a universal region of ITS2 in the ribosomal DNA gene array present in any strongylid nematode, and three different primer/probe sets each targeting a specific region, including the most important and pathogenic genera of GI nematodes of grazing sheep in Sweden (Appendix). The genus specific primers set have a specific forward primer, but uses NC2 (Gasser et al., 1994) as a common reverse primer. The primer/probe sets were ordered online at bio-rad.com/PrimePCR (www.bio-rad.com).