Elsevier

Phytochemistry

Volume 20, Issue 8, 1981, Pages 1781-1784
Phytochemistry

The complete amino acid sequence of a major wheat protein inhibitor of α-amylase

https://doi.org/10.1016/0031-9422(81)84003-4Get rights and content

Abstract

The complete amino acid sequence of one of the major wheat protein iso-inhibitors of α-amylase was determined. The sequence was deduced from analysis of fragments and peptides derived from the protein by cleavage with cyanogen bromide and by digestion with trypsin, chymotrypsin, thermolysin and the Staphylococcus aureus V8 protease. The molecule consists of a single polypeptide chain of 123 residues. Both serine and alanine were found in position 65, and further minor examples of microheterogeneity were observed in four other residues.

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