Biological activity of monoclonal antibodies to operationally defined antigenic regions on the hemagglutinin molecule of A/Seal/Massachusetts/1 /80 (H7N7) influenza virus
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2021, PhytomedicineCitation Excerpt :Titers > 1:1,280 were assigned a titer value of 1:1,280. MN was performed as described previously (Kida et al., 1982), and pre- and post-vaccination serum samples were tested. To determine the neutralizing antibody (NAb) titer, heat-inactivated serum samples were serially diluted twice.
Inactivated whole virus particle vaccine with potent immunogenicity and limited IL-6 induction is ideal for influenza
2019, VaccineCitation Excerpt :One vaccine manufacturer also produced a quadrivalent vaccine containing viruses with the surface antigens of A/California/07/2009 (H1N1), A/Hong Kong/4801/2014 (H3N2), B/Phuket/3073/2013 (Yamagata), and B/Texas/2/2013 (Victoria). Vaccine virus strains were propagated in embryonated chicken eggs and highly purified from the allantoic fluids through sucrose density gradient centrifugation [20] that had not being applied for the preparation of the old WPVs until the time when influenza vaccine for human use was changed to ether-split one in 1972 in Japan. In the present study, WPVs were prepared from the highly purified virions by inactivation with formalin and/or β-propiolactone according to the standard methods used by each vaccine manufacturer.
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Present address: Department of Hygiene and Microbiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, 060 Japan.