[2] Preparation of myoglobins
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X-ray fluorescence holography of biological metal sites: Application to myoglobin
2022, Biochemical and Biophysical Research CommunicationsBiophysical and mass spectrometry based characterization of methylglyoxal-modified myoglobin: Role of advanced glycation end products in inducing protein structural alterations
2021, International Journal of Biological MacromoleculesFunctional inhibition of redox regulated heme proteins: A novel mechanism towards oxidative stress induced by homocysteine
2021, Redox BiologyCitation Excerpt :Preparation of protein stock solutions and determination of concentrations- Protein solutions were dialyzed extensively against 0.1 M KCl solution at 4 °C and filtered using Millipore syringe filter (0.22 μm). Concentrations of protein solutions were determined using ϵ, molar extinction coefficient values of 39000 M−1 cm−1 at 280 nm for Lyz [20], 9800 M−1cm−1 at 277.5 nm for RNase-A [21], 1,05,000 M−1cm−1 at 405 nm for HRP [22], 1,09,000 M−1cm−1 at 409 nm for Cyt c [23], 1,79,000 M−1cm−1 at 405 nm for Hb [24] and 1,16,000 at 408 nm for Myo [25]. All solutions for optical measurements were prepared in degassed 0.05 M potassium phosphate buffer (pH 7.4) containing 0.1 M KCl.
Long-term incubation of myoglobin with glyoxal induces amyloid like aggregation of the heme protein: Implications of advanced glycation end products in protein conformational disorders
2021, Journal of Molecular LiquidsCitation Excerpt :All other reagents were AR grade and purchased locally. Purchased Mb which is mostly in met, deoxy form, was dissolved in 50 mM potassium phosphate buffer (PB), pH 6.0, and concentration determined from its Soret absorbance using ε408 nm = 116 mM−1 cm−1 (monomer basis) [49,50,52–56]. Both Mb and glyoxal solutions were sterile-filtered and sterile conditions were maintained during incubation of the samples.
Methylglyoxal-induced modification causes aggregation of myoglobin
2016, Spectrochimica Acta - Part A: Molecular and Biomolecular SpectroscopyCitation Excerpt :All other reagents were AR grade and purchased locally. Mb was dissolved in 50 mM potassium phosphate buffer (PB), pH 6.0, and concentration was determined considering ε408 nm = 116 mMˉ1 cmˉ1 [37]. The stock concentration of MG in water was estimated using ε284 nm = 12.3 Mˉ1 cmˉ1 [38].