Cell
ArticleEvidence for transposition of dispersed repetitive DNA families in yeast
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2023, Algal ResearchGenome Assembly of the Ty1-Less Saccharomyces paradoxus Strain DG1768
2022, Microbiology Resource AnnouncementsInnovative Tools and Strategies for Optimizing Yeast Cell Factories
2021, Trends in BiotechnologyCitation Excerpt :In addition, catalytically dead Cas9 (dCas9)-mediated Target-AID (vertebrate activation-induced cytidine deaminase), a synthetic hybrid complex that performs highly efficient C to G and C to T mutations, was successfully used for high-throughput loss-of-function screens [47], and provides an attractive method to screen yeast strains for de novo features. Furthermore, the σ sequences, which are a family of repetitive DNA sequences (at least 100 copies) in the S. cerevisiae genome [48], were used together with the CRISPR/Cas9 system in a novel method of genome shuffling [49]. Cas9, guided by a gRNA targeting the σ sequences, cleaves DNA at multiple sites, thus promoting endogenous DNA repair and mutagenesis [50], which can lead to improvement of yeast characteristics when cultivated under specific conditions (e.g., thermotolerant S. cerevisiae can be obtained under high-temperature conditions [49]).
The biochemical and genetic discovery of the SAGA complex
2021, Biochimica et Biophysica Acta - Gene Regulatory MechanismsCitation Excerpt :At the time, it was not envisioned that the mutants would identify conserved transcription factors and chromatin proteins. Ty transposable elements of S. cerevisiae are a family of heterogeneous elements that are structurally and genetically similar to mammalian proviruses [39–44]. Early studies revealed that Ty elements conferred mutant phenotypes when they transposed into the regulatory regions of genes [43,45–47].
Systematically redesigning and optimizing the expression of <inf>D</inf>-lactate dehydrogenase efficiently produces high-optical-purity <inf>D</inf>-lactic acid in Saccharomyces cerevisiae
2019, Biochemical Engineering JournalCitation Excerpt :The yeast transposable element Tyl is one such family of repeated sequences and comprises a 5.6-kb sequence, which includes a non-inverted 0.38-kb sequence of another repetitive family “δ” at each end. Particularly, there were about 35 copies of Ty1 per genome, and the transposable element Ty1 had an effect on the pattern of the genetic expression in the yeast cells [26,27]. In this work, a synthetic biology approach was used to construct high-producing D-LA strains; this approach was based on selecting a combination of a suitable promoter, a terminator, and the D-LDH gene.