A construction is given for difference sets in certain non-cyclic groups with the parameters , , , n = q2s for every prime power q and every positive integer s. If qs is odd, the construction yields at least inequivalent difference sets in the same group. For q = 5, s = 2 a difference set is obtained with the parameters (v, k, λ, n) = (4000, 775, 150, 625), which has minus one as a multiplier.