PaperStrain and process for production of polygalacturonase
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2019, Enzyme and Microbial TechnologyCitation Excerpt :Different organic solvents and salts were used as precipitants (acetone, propanol, ethanol, ammonium sulphate) and the best solvent was selected for precipitation. Pectinase activity in free and m-combi CLEA was determined using polygalacturonic acid as the substrate according to the method described earlier [18]. Appropriate aliquots of free and immobilized enzymes(containing 25U/mg each) were mixed with 0.5 ml of 0.5% polygalacturonic acid and final volume was made to 1.5 ml with 50 mM sodium acetate buffer pH 5, all the samples were incubated for 10 min at 30 °C and reducing sugars were estimated using dinitrosalicylic acid method.
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2017, Physiological and Molecular Plant PathologyCitation Excerpt :In some host-pathogen interactions, these enzymes are thought to be the determinants of virulence [12] and also disrupt other systems of the host [13,14]. Apart from cell wall degrading enzymes secreted by a wide variety of saprophytic and phytopathogenic microorganisms [15], most pathogens produce more cellulolytic than pectolytic enzymes [16]. Lipases and proteases are the important enzymes in pathogenesis, which attack plasmalemma after degradation of cell wall by proteases along with pectolytic and cellulolytic enzymes [17].
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2017, International Journal of Biological MacromoleculesPectinase production by Aspergillus niger LB-02-SF is influenced by the culture medium composition and the addition of the enzyme inducer after biomass growth
2017, Process BiochemistryCitation Excerpt :From this moment on, the level of dissolved oxygen dropped significantly until 30% of saturation and remained at this value for almost 60 h, starting at the 18th hour of cultivation, when the stirring speed was automatically increased to near the operational limit (750 rpm) (Fig. 2A). Filamentous fungal cells may be damaged by shear stress when excessive stirring speeds are used, which affects growth and metabolite production [43–46]. The cultivation in medium M induced a sharp decrease in biomass starting at 100 h and was less than 4 g/L at the end of the experiment (Fig. 1A).
Dilute acid pretreatment and fermentation of sugar beet pulp to ethanol
2013, Applied EnergyCitation Excerpt :The activities of cellulase and β-glucosidase were measured on a FPU and CBU basis, respectively [19]. The pectinase activity, PGU, was determined following the methods developed by Bailey and Pessa [20] and Dalal et al. [21]. The significance levels of different treatments were determined by analysis of variance (ANOVA) and least significant difference (LSD) (α = 0.05 and pcritical = 0.05).