Confocal imaging of ionised calcium in living plant cells

doi:10.1016/0143-4160(90)90006-G

Cell Calcium

Volume 11, Issue 4, April 1990, Pages 291-297

https://doi.org/10.1016/0143-4160(90)90006-G Get rights and content

Abstract

Laser-scanning confocal microscopy has been used in conjunction with Fluo-3, a highly fluorescent visible wavelength probe for Ca²⁺, to visualise Ca²⁺-dynamics in the function of living plant cells. This combination has overcome many of the problems that have limited the use of fluorescence imaging techniques in the study of the role of cations (Ca²⁺ and H⁺) in plant cell physiology and enables these processes to be studied in single cells within intact plant tissue preparations. Maize coleoptiles respond to application of ionophores and plant growth hormones with elevations in cytosolic Ca²⁺ that can be resolved with a high degree of spatial resolution and can be interpreted quantitatively.

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Confocal imaging of ionised calcium in living plant cells

Abstract

Cell Calcium

Cell Calcium

J. Biol. Chem.

Cell Calcium

Cell Calcium

Regional changes in Ca2+ underlying the contraction of single smooth muscle cells

Science

[Ca2+]i in single isolated cardiac cells: a review of recent results obtained with digital imaging microscopy and fura-2

Can. J. Physiol. Pharmacol.

Electrically and chemically mediated increases in intracellular calcium in neuronal growth cones

J. Neurosci.

Transition from metaphase to anaphase is accompanied by local changes in cytoplasmic free calcium in Pt K2 kidney epithelial cells

Problems with the application of quin-2AM to measuring free calcium in plant cells

Plant Cell Environ.

A gradient of cytoplasmic free calcium in growing rhizoid cells of Fucus serratus

Nature

Cytoplasmic calcium and α-amylase secretion from barley aleurone protoplasts

Eur. J. Cell Biol.

Regional changes in Ca²⁺ underlying the contraction of single smooth muscle cells

[Ca²⁺]_i in single isolated cardiac cells: a review of recent results obtained with digital imaging microscopy and fura-2

Problems with the application of $quin- 2 AM$ to measuring free calcium in plant cells