Optimal conditions for in vitro blastogenesis of feline peripheral blood lymphocytes
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2012, Veterinary Immunology and ImmunopathologyCitation Excerpt :Determination of lymphocyte proliferation to mitogens, specific antigen or specific allergen is applicable in both research and clinical settings for the study of congenital and acquired immune defects and response to therapeutics as it reflects the ability of lymphocytes to respond to external signals. Established techniques for lymphocyte proliferation in the cat use incorporation of a radioactive nucleoside ([3H]thymidine) into cellular DNA (Than et al., 1982) or, to avoid radioactivity, commercially available ELISA kits can be used which incorporate bromodeoxyuridine (BrdU) with a chromogenic reaction (Reinero et al., 2006). Radioactive assays, while having the advantage of high sensitivity, are a biological hazard requiring special licensure and secured facilities.
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