Elsevier

Antiviral Research

Volume 19, Issue 3, September 1992, Pages 219-232
Antiviral Research

The activity of (S)-1-[(3-hydroxy-2-phosphonyl methoxy) propyl] cytosine (HPMPC) against equine herpesvirus-1 (EHV-1) in cell cultures, mice and horses

https://doi.org/10.1016/0166-3542(92)90081-FGet rights and content

Abstract

The activity of the nucleotide analogue, (S)-1-[(3-hydroxy-2-phosphonyl methoxy) propyl] cytosine (HPMPC), against equine herpesvirus-1 (EHV-1) was tested in cell culture, mice and foals. The ED50 for plaque reduction was found to be 0.07 and 0.03 μg/ml in RK-13 and EEL cells respectively. In mice, a single administration of HPMPC (20 mg/kg, s.c.) was very effective at reducing clinical signs and virus replication if given on the day before intranasal inoculation with EHV-1. Treatment on the day of infection or day 1 p.i. was less effective, but still significantly reduced clinical signs and virus titres in the target organs (lungs and nasal tissue). Furthermore, HPMPC was found to protect mice partially from an intracerebral inoculation with EHV-1. Experiments in the horse suggested that HPMPC was also very active against EHV-1 in the natural host. Thus a single administration of HPMPC at 20 mg/kg, s.c., on the day of infection, markedly reduced clinical signs and nasal excretion of virus following intranasal inoculation with EHV-1. HPMPC given as a divided dose of 1 mg/kg on day 0 and day 3 p.i. had no effect on clinical signs but did reduce nasal excretion of virus. The significance of these results is discussed.

References (24)

  • E. De Clercq et al.

    A novel selective broad-spectrum anti-DNA virus agent

    Nature

    (1986)
  • E. De Clercq et al.

    Antiviral activity of phosphonylmethoxyalkyl derivatives of purine and pyrimidines

    Antiviral Res.

    (1987)
  • Cited by (21)

    • Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: Efficacy of decitabine and valganciclovir alone or in combination

      2020, Antiviral Research
      Citation Excerpt :

      E. Derm cells and EEK cells are both equine cell lines and most adapted to identify new antiviral compounds in equid species, especially EEK that was derived from a horse foetus that is one of the target of EHV-1 (Léon et al., 2008; Smith et al., 2010). Even though RK13 cells are not equine cells, they have been most frequently used in EHV1 antiviral studies (Azab et al., 2010; de la Fuente et al., 1992; Gibson, 1992; Rollinson, 1987). All compounds except aciclovir and gemcitabine showed some consistent antiviral activity in the three different cell lines.

    • Antiherpetic Drugs in Equine Medicine

      2017, Veterinary Clinics of North America - Equine Practice
      Citation Excerpt :

      Although cidofovir is administered as a 1-hour infusion in humans, its neutral pH has allowed for subcutaneous administration in other species. Consequently, cidofovir has also been administered subcutaneously to several foals, but pharmacokinetic studies in horses are lacking.92 Idoxuridine and trifluridine are structurally related thymidine analogs that inhibit DNA synthesis.

    • Viral respiratory disease in athletic horses

      2013, Equine Sports Medicine and Surgery: Second Edition
    View all citing articles on Scopus
    View full text