Short sequence-paper
Cloning and expression analysis of a cytochrome P-45011β cDNA in sheep

https://doi.org/10.1016/0167-4781(94)00190-EGet rights and content

Abstract

A full length ovine steroid 11β-hydroxylase (cytochrome P-45011β) cDNA clone from a sheep adrenal cortex cDNA library was isolated. Sequence analysis indicates that this cDNA clone resembles bovine P-45011β cDNA (95% nucleotide sequence homology) more closely than rat P-45011β cDNA (69% nucleotide sequence homology). Although the levels of nucleotide sequence homology of this cDNA clone to the rat P-45011β cDNA and the rat P-450aldo cDNA are similar, the putative amino acid sequence shows a closer resemblance to rat P-450aldo protein. Northern blot analysis shows that there are three sizes of transcript and they are expressed throughout the adrenal cortex.

References (28)

  • M. Lauber et al.

    Arch. Biochem. Biophys.

    (1989)
  • T. Ogishima et al.

    J. Biol. Chem.

    (1989)
  • R.J. Crawford et al.

    J. Biol. Chem.

    (1992)
  • Y. Nonaka et al.

    FEBS Lett.

    (1989)
  • M. Imai et al.

    FEBS Lett.

    (1990)
  • Y. Nonaka et al.

    J. Steroid Biochem. Mol. Biol.

    (1992)
  • C.J.P. Giroud et al.
  • P.J. Ayres et al.

    Biochem. J.

    (1956)
  • T. Ohnishi et al.

    J. Steroid Biochem.

    (1988)
  • T. Kawamoto et al.
  • L.J. Domalik et al.

    Mol. Endocrinol.

    (1991)
  • K.-I. Morohashi et al.

    J. Biochem.

    (1990)
  • T. Ogishima et al.

    J. Biochem.

    (1989)
  • L.M. Braley et al.

    Endocrinology

    (1992)
  • Cited by (0)

    The cDNA and putative amino acid sequence data reported in this paper have been submitted to the EMBL/GenBank Data Libraries under the accession number L34337.

    View full text