Molecular cloning and sequencing of the gene encoding a sheep arginine vasopressin type 1a receptor

doi:10.1016/0167-4781(95)00125-Z

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

Volume 1263, Issue 3, 19 September 1995, Pages 266-270

https://doi.org/10.1016/0167-4781(95)00125-Z Get rights and content

Abstract

The gene for the sheep arginine vasopressin type 1a (V_1a) receptor subtype was cloned from a genomic library. The deduced amino acid sequence shows characteristics of a G-protein coupled receptor and high sequence identity to human and rat V_1a receptor sequences (81% and 73%, respectively). Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed a tissue distribution consistent with a type V_1a receptor. The genomic DNA (7.1 kb) contains a 1586 bp intron between the putative 6th and 7th transmembrane domains.

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Genomic characterization of the sheep vasopressin V1a receptor gene and promoter, with assignment to bands q23-24 of sheep chromosome 3 and cattle chromosome 5
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Arginine vasopressin interacts with the vasopressin type 1a receptor (V1aR) to initiate physiological effects such as vasoconstriction of blood vessels and glycogenolysis. AVP is also involved in central nervous effects such as body homeostasis and blood pressure control. The complete genomic organization of the sheep V1aR gene has been determined, including the presence of one major and two minor transcriptional start sites at −321, −206 and −91 bp respectively, relative to the ATG codon. Another more distal minor transcriptional start site was also localized between nucleotides −997 and −892 relative to the ATG codon. One intron exists in the sheep V1aR gene and potential cis- and trans- acting sites were identified in the sheep V1aR promoter. The promoter was also compared to the rat V1aR promoter. The sheep V1aR promoter displays features typical of housekeeping genes, although tissue-specific expression does not support this. V1aR mRNA is absent in the adult sheep liver but not the kidney. One copy of the V1aR gene exists in the sheep genome, which was localized to chromosome 3q23–24, and to the homoeologous position, 5q23–24 in cattle.
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: The nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank Data Libraries under the accession number L41502.

¹: Present address: Department of Pathology, Peter MacCallum Cancer Research Institute, Heidelberg, Victoria, Australia.

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Molecular cloning and sequencing of the gene encoding a sheep arginine vasopressin type 1a receptor

Abstract

J. Biol. Chem.

J. Biol. Chem.

J. Mol. Biol.

Comp. Biochem. Physiol.

Biochem. Biophys. Res. Commun.

Anal. Biochem.

Biochem. Soc. Trans.

Nature