Stimulation-induced uptake of horseradish peroxidase by rat cortical synapses

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Abstract

Horseradish peroxidase has been utilised as an extracellular tracer in the study of membrane dynamics in synapses of rat cerebral cortex. Stimulation of the presynaptic terminal with 50 mM K+ resulted in a slight vesicular depletion and an increase in terminal circumference, with no significant change in area. Subsequent resting of the tissue for 15 min resulted in recovery of vesicle number and an increase in the number of labelled vesicles and vacuolar profiles within the presynaptic terminal. It is suggested that vacuoles may be intimately involved in the recovery of vesicle membrane from the plasmalemma following high frequency synaptic transmission.

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    Citation Excerpt :

    In the early 1970s experiments using exogenous labels, for instance HRP applied to nerve terminal areas, could demonstrate that the applied peroxidase was endocytosed by the nerve terminals, incorporated into transport organelles, and carried back to the nerve cell body (e.g. Kristensson and Olsson, 1971, 1973; LaVail and LaVail, 1974; Ellison and Clark, 1975). The degree of endocytosis was found to depend on the impulse activity in the neuron, the higher frequency of firing the greater the uptake (e.g. Heuser and Reese, 1972; Cooke et al., 1975; Dolivo et al., 1977). This uptake in the nerve terminal area is coupled to the release of transmitter and the recycling of synaptic vesicles during activity (Heuser and Reese, 1972).

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