Localization of preprogalanin mRNA in rat brain: In situ hybridization study with a synthetic oligonucleotide probe

doi:10.1016/0304-3940(90)90570-Y

Neuroscience Letters

Volume 114, Issue 3, 13 July 1990, Pages 241-247

https://doi.org/10.1016/0304-3940(90)90570-Y Get rights and content

Abstract

Galanin is a peptide containing 29 amino acid residues that is present in both the central and peripheral nervous systems. Galanin has multiple putative biological functions including regulation of hormone release, stimulation of feeding behaviour, and effects on blood pressure. This study examined the distribution of neurones expressing preprogalanin mRNA in the rat brain by in situ hybridization of a specific ³⁵S-labelled oligonucleotide. Preprogalanin mRNA was detected in several regions of brain, with high concentrations in the paraventricular, periventricular, supraoptic, dorsomedial and arcuate nuclei of the hypothalamus; the locus coeruleus and dorsal raphe nucleus in the pons; and the nucleus tractus solitarii and ventrolateral reticular nucleus in the medulla. These findings are consistent with studies of the cellular localization of galanin-like immunoreactivity in rat brain, and further suggest the involvement of galanin in the regulation of several functions ranging from water balance to blood pressure control.

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^∗: Present address: Laboratory of Molecular Neuroendocrinology, ZMBH, University of Heidelberg, INF 282, D-6900 Heidelberg, F.R.G.

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Localization of preprogalanin mRNA in rat brain: In situ hybridization study with a synthetic oligonucleotide probe

Abstract

Neuroscience

Brain Res.

Neuroscience

Eur. J. Pharmacol.