BBA Report
A factor synthesized by rabbit periosteal fibroblasts stimulates bone resorption and collagenase production by connective tissue cells in vitro

https://doi.org/10.1016/0304-4165(84)90409-4Get rights and content

Abstract

Unstimulated monolayer cultures of confluent rabbit periosteal fibroblasts synthesize a factor that stimulates bone resorption in vitro. Furthermore it stimulates rabbit chondrocytes and mouse osteoblasts to synthesize collagenase. The factor has no effect on dead bone in culture, and its activity on live bone is mediated principally by osteoclasts, since it is 75% inhibited by salmon calcitonin. Characterization of the factor by gel filtration and isoelectric focusing indicates an Mr in the range 15 000–25 000 and a pI corresponding to approx. pH 4.7. These biological and physicochemical properties are similar to those reported for a factor released by peripheral blood monocytes. However, whereas human monocyte factor in both the crude and partially-purified state exhibits interleukin-1 activity, crude and fractionated periosteal fibroblast-conditioned medium does not. This is the first report of a conditioned medium containing a molecule like the monocyte-factor which appears to have no interleukin 1 activity. The factor may be synthesized by a wide range of cell types, and could have an important role in mediating connective tissue degradation during both physiological and pathological resorption.

References (34)

  • G.A. Howard et al.

    Metab. Bone Dis. and Rel. Res.

    (1980)
  • R.H. Drivdahl et al.

    Biochim. Biophys. Acta

    (1982)
  • U. Trechsel et al.

    Biochim. Biophys. Acta

    (1982)
  • M. Gowen et al.

    Biochim. Biophys. Acta

    (1983)
  • J.J. Oppenheim et al.

    Immunol. Today

    (1982)
  • Y. Okai et al.

    FEBS Lett.

    (1982)
  • S.J. Duncan et al.

    Biochem. Pharmacol.

    (1983)
  • T.E. Cawston et al.

    Anal. Biochem.

    (1979)
  • G. Murphy et al.

    Biochim. Biophys. Acta

    (1977)
  • W.H. Harris et al.

    N. Engl. J. Med.

    (1969)
  • E.R. Thompson et al.

    Endocrinology

    (1975)
  • J.E. Puzas et al.
  • E. Canalis et al.

    Science

    (1980)
  • R.H. Drivdahl et al.
  • M.R. Urist et al.

    Clin. Orthop.

    (1982)
  • J.R. Farley et al.

    Biochemistry

    (1982)
  • J.R. Farley et al.

    Biochemistry

    (1982)
  • Cited by (0)

    View full text