Elsevier

Scientia Horticulturae

Volume 24, Issue 2, November 1984, Pages 123-134
Scientia Horticulturae

In vitro propagation of some olive (Olea europaea sativa L.) cultivars with different root-ability, and medium development using analytical data from developing shoots and embryos

https://doi.org/10.1016/0304-4238(84)90143-2Get rights and content

Abstract

Short- and long-term objectives for research on tissue culture of the olive are described. Sterile shoots were obtained from single-node woody explants or buds of 3 olive cultivars (‘Frantoio’, ‘Dolce Agogia’ and ‘Moraiolo’) with different root-ability, collected from shoots having different degrees of juvenility (suckers, vigorous nonfruit-bearing and fruit-bearing shoots, which are easy, medium and difficult to root, respectively).

Because many of the media tested did not give a satisfactory growth rate and good quality shoots, a new medium was formulated by comparing data from analysis of the main mineral elements found in the apical shoots (4–5 mm long) and in mature embryos in olive and almond. Olive tissues were characterized by a high content of Ca, Mg, S, Cu and Zn compared to almond, which is easy to propagate on MS medium. In this newly derived medium, characterized by a high content of these elements, multiplication rate (number of nodes formed per explant) was about 9× in 40 days. The shoots grew more rapidly and were more tender than when grown in other media. Washing of the explants in water or GSH (reduced glutathione) solution, before sub-culturing, improved quality and growth rate of the shoots.

Explants, with 2 or 3 nodes, rooted easily in half-strength MS, in Bourgin and Nitsch, or in half Knop macro and Heller microelements, agar media, with 1 mg 1−1 NAA and 2% sucrose. Rooting was not affected by the different degrees of juvenility of the original explants used.

Hardening-off was achieved by growing plants in a 1:1 mixture of perlite and peat-moss in a transparent plastic chamber with saturated circulating air for 1 month. GA3 sprayed on the leaves was found to be beneficial in stimulating growth resumption of plantlets.

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