The Drosophila melanogaster genome contains a member of the Rh/T2/S-glycoprotein family of ribonuclease-encoding genes

doi:10.1016/0378-1119(94)00896-Z

Gene

Volume 158, Issue 2, 9 June 1995, Pages 203-207

https://doi.org/10.1016/0378-1119(94)00896-Z Get rights and content

Abstract

Members of the Rh/T₂/S-glycoprotein family of ribonuclease(RNase)-encoding genes have been found predominantly in fungi, plants and bacteria, where they have been implicated in functions as diverse as the phosphate-starvation response and self-incompatibility. We report the isolation and sequence of DmRNase-66B, the first member of this family to be found in an insect genome. This gene was identified by the analysis of a cDNA clone derived from cytological region 66B1–2 of the genome of Drosophila melanogaster. In a search of sequence databases for homologs of this gene, two animal viral proteins, gp53 of the bovine viral diarrhea virus (BVDV) and gp44/48 of the hog cholera virus (HCV), were also found to exhibit the characteristic features of this class of RNases. In all cases, the proteins contain two conserved pentameric amino-acid regions that have been shown to lie in the active site of these RNases. A series of Cys residues are also conserved in all members of this gene family. The discovery of members of this family of genes in an insect genome indicates that these RNases are widely conserved and play important roles in the animal, as well as the plant and prokaryotic kingdoms.

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^∗: Present addresses: (L.P.) Department of Genetics, University of Adelaide, Adelaide, SA 5005, Australia. Tel. (61-8) 303-4043; (G.H.) Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305-5427, USA. Tel. (1-415) 723-9703

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Short communicationThe Drosophila melanogaster genome contains a member of the Rh/T2/S-glycoprotein family of ribonuclease-encoding genes

Abstract

J. Mol. Biol

FEBS Lett.

FEBS Lett.

J. Mol. Biol

Gene

Self-incompatibility in Petunia inflata: isolation and characterization of complementary DNA encoding three S-allele-associated proteins

Cloning of cDNA for a stylar glycoprotein associated with expression of self-incompatibility in Nicotiana alata

Nature

Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata

Plant Cell

Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates

Nucleic Acids Res.

Sequence variability and developmental expression of S-alleles in self-incompatible and pseudo-self-compatible petunia

Plant Cell

Zygotic expression of the pebble locus is required for cytokinesis during the postblastoderm mitoses of Drosophila

Development

Primary structure of a base nonspecific ribonuclease from Rhizopus niveus

J. Biochem

Polymorphism at the self-incompatibility locus in Solanaccae predates speciation

Amino-acid sequence of an extracellular, phosphate-starvation induced ribonuclease from cultured tomato (Lycopersicon esculentum) cells

Eur. J. Biochem

The pebble gene is required for cytokinesis in Drosophila

J. Cell Sci.

Phosphate-regulated induction of intracellular ribonucleases in cultured tomato (Lycopersicon esculentum) cells

Plant Physiol

Short communication
The Drosophila melanogaster genome contains a member of the Rh/T₂/S-glycoprotein family of ribonuclease-encoding genes