Direct detection of cell surface receptors for glycosyl residues on Phytophthora cinnamomi using polysaccharide-coated fluorescent latex beads

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Abstract

We have confirmed the presence of a fucose-specific binding protein on the surface of zoospores, cysts and germinated cysts of Phytophthora cinnamomi Rands using a direct binding assay with polysaccharide-coated fluorescent latex beads. The specificity for fucose was established using a range of polysaccharides and proteoglycans. Only maize root slime- and fucoidan-coated beads bound to the surface of zoospores, cysts and germinated cysts. Binding could be blocked with fucose or fucose-containing polysaccharides. In addition, maize root slime-coupled beads which were pre-incubated with the fucose-specific lectin from Ulex europeaus (UEA-1) did not bind to the cell surface of fungal propagules.

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    This work was supported by a Commonwealth Government Special Research Centre Grant.

    Present address: The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, Victoria 3050, Australia.

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