Hydroxylamine addition enhances fast recovery of anammox activity suffering Cr(VI) inhibition

doi:10.1016/j.biortech.2021.124920

Bioresource Technology

Volume 329, June 2021, 124920

https://doi.org/10.1016/j.biortech.2021.124920 Get rights and content

Highlights

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NH₂OH recovered the Cr(VI) inhibition on anammox activity, with recovery over 150%.
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NH₂OH relieved Cr(VI) inhibition on anammox due to the reduction to Cr(III).
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1 ~ 2 mg N/L NH₂OH accelerated the recovery of reactor performance with 2.84 folds.
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The EPS, activity and heme content are better recovered with NH₂OH addition.

Abstract

Hydroxylamine (NH₂OH), one of the most important intermediates of anammox was employed to test the recovery performance because of its stimulation to anammox bacteria. Batch test indicated simultaneous addition of 1.83 ~ 9.17 mg N /L NH₂OH relieved Cr(VI) inhibition because of extracellular reduction to Cr(III). The recovery efficiency (RE) was over 166%, with the effluent Cr(VI) and Cr(III) below 0.25 and 0.12 mg/L, respectively. Anammox activity after Cr(VI) inhibition was effectively recovered by 8 mg N/L NH₂OH with RE at 218%. The long-term operation showed 1 ~ 2 mg N/L NH₂OH accelerated the recover speed of nitrogen removal rate with 2.84 folds, as well as improving NH₄⁺ conversion ratio and reducing NO₃⁻ production. After 55 days recovery, extracellular polymeric substance concentration, anammox activity and heme content recovered better with NH₂OH addition. This study will provide the theoretical basis for rapid recovery of anammox activity by NH₂OH when suffering Cr(VI) inhibition.

Graphical abstract

Section snippets

Instruction

Anaerobic Ammonia Oxidation (anammox) presents excellent performance in energy conservation and environmental protection (Tan et al., 2020). But the sensitivity limits its application in practical wastewater treatment. Typically, the high-concentration ammonia wastewater produced by many industries is often accompanied by a variety of heavy metals. The presence of heavy metals will damage the activity of anammox bacteria and the nitrogen removal performance. Among which, Cr presents strong

Experimental setup and inoculation sludge

The working volume of Up-flow Anaerobic Sludge Blanket Reactor (UASB) is 1 L. The NRR was 8.0 ~ 10.3 kg/m³/d, and the specific anammox activity (SAA) was 0.28 kg N/kg VSS/d. The results from 16SrRNA sequencing showed that Candidatus Brocadia was the dominant species, with the relative abundance of 40%. Sludge were 2.5 ~ 5.0 mm in diameter, with an average diameter of 3.95 mm. The sludge volume index (SVI), SVI₅ and SVI₃₀ were 22.95 and 20.15, respectively. The concentration of volatile

Simultaneous NH₂OH addition relieves Cr(VI) inhibition

The Cr(VI) inhibition on anammox biomass was initially investigated with batch tests. A non-competitive inhibition model (Eq. (3)) was used to fit the relationship between Cr(VI) concentration and IP of anammox activity. As shown in Fig. 1a, the IC₅₀ of Cr(VI) on anammox was 6.8 mg/L (0.13 mmol/L). Specifically, the addition of Cr(VI) at this concentration making the corresponding SAA decreased from 0.28 kg N/kg VSS/d to 0.14 kg N/kg VSS/d. According to the inhibition model, when Cr(VI)

Conclusions

•
NH₂OH relieves Cr(VI) inhibition on anammox activity because of extracellular reduction of Cr(VI) to Cr(III). The RE was over 166% with NH₂OH addition.
•
NH₂OH recovers anammox activity after Cr(VI) inhibition. The best recovery performance of SAA was obtained when NH₂OH was added at 8 mg N/L with RE at 218%.
•
The addition of NH₂OH possessed a significant strengthening effect on ammonium removal. The NRR recovery rate increased 184% after NH₂OH addition.
•
Long-term recovery of anammox activity

CRediT authorship contribution statement

Fan Feng: Investigation, Writing - original draft, Writing - review & editing. Xi Tang: Investigation, Writing - original draft, Writing - review & editing. Caiyan Qu: Investigation, Methodology. Xuan Lu: Investigation, Methodology. Zhigong Liu: Visualization. Jia Tang: Visualization. Chong-Jian Tang: Investigation, Writing - review & editing. Liyuan Chai: Writing - review & editing.

Declaration of Competing Interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Acknowledgments

This work is partially supported by the National Natural Science Foundation of China (51878662, 52000182), the Key Project of Science and Technology Program of Hunan Province (2017SK2420), the Fundamental Research Funds for the Central Universities of Central South University (No.2017zzts599). Dr. Tang C.-J. is supported by the Distinguished Youth Program of the Natural Science Foundation of Hunan Province (2019JJ20033).

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¹: Authors contributed equally to the paper.

View full text

Hydroxylamine addition enhances fast recovery of anammox activity suffering Cr(VI) inhibition

Highlights

Abstract

Graphical abstract

Section snippets

Instruction

Experimental setup and inoculation sludge

Simultaneous NH2OH addition relieves Cr(VI) inhibition

Conclusions

CRediT authorship contribution statement

Declaration of Competing Interest

Acknowledgments

Process Biochem.

J Environ Sci.

Sci Total Environ.

Bioresour Technol.

J Biosci Bioeng.

Bioresour Technol.

Bioresour Technol.

Sci Total Environ.

Water Res.

Biochem Eng J.

Sci Total Environ.

Water Res.

Chem Eng J.

Water Res.

Water Res.

Simultaneous NH₂OH addition relieves Cr(VI) inhibition