Review
Trabecular meshwork stiffness in glaucoma

https://doi.org/10.1016/j.exer.2016.07.011Get rights and content

Highlights

  • Trabecular meshwork stiffness may be an important contributor to raised IOP.

  • Measurements of trabecular meshwork (TM) stiffness, and factors affecting this quantity, are reviewed.

  • A new technique to directly measure TM stiffness in mouse eyes is presented.

  • Preliminary data relating TM stiffness and outflow facility in C57BL/6J and CBA/J mice are shown.

Abstract

Alterations in stiffness of the trabecular meshwork (TM) may play an important role in primary open-angle glaucoma (POAG), the second leading cause of blindness. Specifically, certain data suggest an association between elevated intraocular pressure (IOP) and increased TM stiffness; however, the underlying link between TM stiffness and IOP remains unclear and requires further study. We here first review the literature on TM stiffness measurements, encompassing various species and based on a number of measurement techniques, including direct approaches such as atomic force microscopy (AFM) and uniaxial tension tests, and indirect methods based on a beam deflection model. We also briefly review the effects of several factors that affect TM stiffness, including lysophospholipids, rho-kinase inhibitors, cytoskeletal disrupting agents, dexamethasone (DEX), transforming growth factor-β2 (TGF-β2), nitric oxide (NO) and cellular senescence. We then describe a method we have developed for determining TM stiffness measurement in mice using a cryosection/AFM-based approach, and present preliminary data on TM stiffness in C57BL/6J and CBA/J mouse strains. Finally, we investigate the relationship between TM stiffness and outflow facility between these two strains. The method we have developed shows promise for further direct measurements of mouse TM stiffness, which may be of value in understanding mechanistic relations between outflow facility and TM biomechanical properties.

Introduction

Elevated intraocular pressure (IOP) due to increased resistance to aqueous humor outflow within the conventional outflow pathway is an important risk factor for glaucoma (Gordon et al., 2002, Grant, 1951). The principal site of outflow resistance in this pathway is the trabecular meshwork (TM), including the inner wall of Schlemm's canal (SC) (Maepea and Bill, 1992, Overby et al., 2009, Stamer and Acott, 2012). However, the exact mechanism of how aqueous outflow resistance increases in glaucoma has remained elusive. A better understanding of the pathophysiology of aqueous humor drainage through the TM would be of great benefit to the development of IOP-lowering therapies for glaucoma patients.

In this context, there are several observations which suggest that TM stiffness may be important in ocular hypertension associated with glaucoma. For example, pharmacologic modulation of TM cell actomyosin tone has a significant effect on outflow facility (see below). Further, using direct measurements of TM biomechanical properties, Last et al. (2011) reported that the compressive stiffness of TM was 20 times greater in post mortem glaucomatous human eyes compared to ostensibly healthy eyes. These findings and others reviewed below have motivated studies on the relationship between TM stiffness and ocular hypertension.

Our goal in this paper was to first review the literature on trabecular meshwork stiffness, and to then present a method and preliminary data for directly measuring TM compressive stiffness in mouse eyes. The ability to make such measurements in mice is attractive because it will hopefully allow a more mechanistic understanding of how TM stiffness and fluid flow resistance are inter-related. We begin by introducing some background material on how stiffness is defined and on factors that influence tissue stiffness.

Stiffness is a measure of the tendency of a material to resist deformation when it is loaded, i.e. when a force is applied to it. The extent of tissue deformation can be quantified through the strain, ε; in the simplest case, strain is defined byε=tissuedeformationtissueoriginallength

Similarly, the load is quantified through the stress, σσ=forcetissuecrosssectionalarea

A measure of tissue stiffness is then Young's modulus, E, defined as:E=σ(ε)ε

Here we explicitly note that the stress depends on the strain; in fact, for soft tissues, this dependence is usually non-linear so that Young's modulus is not a constant value, but one that varies with the strain. In this case, we can describe tissue stiffness by an effective Young's modulus, or more simply, by “the modulus”. The implication is that comparison of stiffness values from different studies is strictly only valid when the extent of tissue deformation was comparable between studies.

In addition to the magnitude of strain, the effective Young's modulus of soft tissue typically depends on a number of other factors, including how the external force is applied (i.e. direction, rate of application) and whether the tissue is in tension or compression. Importantly for any discussion of TM stiffness, it should be noted that soft tissues are much softer when they are loaded in compression vs. when they are loaded in tension.

Taking all of the above into consideration, the actual value of the effective Young's modulus should be interpreted as a general indication of tissue stiffness that may not be relevant in all situations. More realistic descriptions of tissue biomechanical behavior require more complex formulations that are beyond the scope of this article (Fung, 1993, Humphrey and Yin, 1987). Nonetheless, measured modulus values are still useful inasmuch as they can be used for relative comparisons of tissue stiffness between samples (e.g. normal vs. glaucomatous) if the testing conditions are identical between samples.

In general, tissue stiffness depends on both cells and extracellular matrix (ECM), and these two components interact in multiple ways in all tissues. Notably, in addition to matricellular signaling pathways and modification of the matrix by the resident cells, it is well known that cells directly sense and respond to the stiffness and topography of their underlying substrate (Discher et al., 2005, Engler et al., 2004, Georges and Janmey, 2005, Russell et al., 2008). For example, fibroblasts change their internal stiffness to try to match that of a stiffer substrate by enhancing actin polymerization and cross-linking (Solon et al., 2007). Similarly, airway smooth muscle cells increase their baseline contractile tone in response to increased substrate stiffness by upregulating their contractile protein expression (West et al., 2011).

Accordingly, we expect the stiffness of human TM to depend in a complex fashion on the resident TM cells, the ECM and the interactions between the two (Fuchshofer and Tamm, 2012, Hoare et al., 2009, Schlunck et al., 2008, Thomasy et al., 2013, Tovar-Vidales et al., 2008). It is known that TM cells are contractile (Lepple-Wienhues et al., 1991) and that elevated outflow resistance can be partly due to an increase in TM tone (Wiederholt et al., 2000). Further, TM cell contraction can direct ECM reorganization, and thus it has been hypothesized that the increased contraction state of TM cells in POAG might be associated with a stiffer TM (Fuchshofer and Tamm, 2012). Additionally, it is suggested that stiffness changes in the ECM of TM may in turn induce alterations in the mechanical properties of the TM cell itself (Last et al., 2011). The biomechanical interaction between cells and ECM, and the likely modulation of this interaction in disease state, make it difficult to “cleanly” determine the relative contributions of cells and ECM to overall TM stiffness and stiffness changes.

Section snippets

Human

The stiffness of human TM has been determined directly and indirectly in several studies. There are currently two approaches for direct measurement: compression (indentation) testing and tensile testing. In view of the Discussion in Section 1.1, modulus values obtained from these two techniques are not expected to be of comparable magnitudes. Atomic force microscopy (AFM) is an example of a compressive testing modality, with the details of the cellular deformation field depending strongly on

Factors and agents affecting TM stiffness

In addition to disease state, there are a number of factors and agents that are known to alter TM stiffness; here, we selectively review several important studies in this area.

Novel measurements of TM stiffness in mice

We here describe preliminary work to develop a new technique for measuring TM stiffness in mice. Eventually we anticipate that this technique will be one part of a strategy designed to mechanistically relate TM stiffness and outflow facility.

Discussion

This preliminary study presents the first method to directly measure the local compressive modulus of mouse TM. We also investigated the correlation between TM stiffness and outflow facility between two mouse strains.

Conclusions and future directions

There is growing evidence suggesting that mechanical properties of TM may be involved in ocular hypertension associated with glaucoma. We know that TM stiffness may be affected by pharmacological agents as well as intrinsic alterations in TM and SC cells. In addition, the ECM may have important effects on TM cellular behavior and, notably, TM cells and their ECM are inextricably linked, with alterations in one affecting the other. By combining the techniques of cryosectioning and AFM, we were

Acknowledgments

We gratefully acknowledge funding support from the Georgia Research Alliance.

References (86)

  • D.R. Overby et al.

    The changing paradigm of outflow resistance generation: towards synergistic models of the JCT and inner wall endothelium

    Exp. Eye Res.

    (2009)
  • K.G. Schwabe et al.

    Ultrastructural and thermocouple evaluation of rapid freezing techniques

    Cryobiology

    (1980)
  • J. Solon et al.

    Fibroblast adaptation and stiffness matching to soft elastic substrates

    Biophys. J.

    (2007)
  • B.D. Stemper et al.

    Mechanics of fresh, refrigerated, and frozen arterial tissue

    J. Surg. Res.

    (2007)
  • M. Tamura et al.

    Development of specific Rho-kinase inhibitors and their clinical application

    Biochimica Biophysica Acta-Proteins Proteomics

    (2005)
  • S.M. Thomasy et al.

    Substratum stiffness and latrunculin B modulate the gene expression of the mechanotransducers YAP and TAZ in human trabecular meshwork cells

    Exp. Eye Res.

    (2013)
  • R. Vargas-Pinto et al.

    The effect of the endothelial cell cortex on atomic force microscopy measurements

    Biophys. J.

    (2013)
  • M. Wiederholt et al.

    The regulation of trabecular meshwork and ciliary muscle contractility

    Prog. Retin Eye Res.

    (2000)
  • T. Boudou et al.

    An extended relationship for the characterization of Young's modulus and Poisson's ratio of tunable polyacrylamide gels

    Biorheology

    (2007)
  • A. Boussommier-Calleja et al.

    Pharmacologic manipulation of conventional outflow facility in ex vivo mouse eyes

    Investig. Ophthalmol. Vis. Sci.

    (2012)
  • A. Boussommier-Calleja et al.

    The influence of genetic background on conventional outflow facility in mice

    Investig. Ophthalmol. Vis. Sci.

    (2013)
  • C. Braunsmann et al.

    Evaluation of lamina cribrosa and peripapillary sclera stiffness in pseudoexfoliation and normal eyes by atomic force microscopy

    Investig. Ophthalmol. Vis. Sci.

    (2012)
  • L.J. Camras et al.

    Differential effects of trabecular meshwork stiffness on outflow facility in normal human and porcine eyes

    Investig. Ophthalmol. Vis. Sci.

    (2012)
  • L.J. Camras et al.

    Circumferential tensile stiffness of glaucomatous trabecular meshwork

    Investig. Ophthalmol. Vis. Sci.

    (2014)
  • L.J. Camras et al.

    Erratum in: Differential effects of trabecular meshwork stiffness on outflow facility in normal human and porcine eyes

    Investig. Ophthalmol. Vis. Sci.

    (2014)
  • M.E. Cavet et al.

    Nitric oxide (NO): an emerging target for the treatment of glaucoma

    Investig. Ophthalmol. Vis. Sci.

    (2014)
  • S.M. Chudgar et al.

    Regulation of connective tissue growth factor expression in the aqueous humor outflow pathway

    Mol. Vis.

    (2006)
  • J. Cortiella et al.

    Influence of acellular natural lung matrix on murine embryonic stem cell differentiation and tissue formation

    Tissue Eng. Part A

    (2010)
  • R. Daneshvar et al.

    Rho-associated kinase inhibitors: potential future treatments for glaucoma

    J. Ophthalmic Vis. Res.

    (2014)
  • D.E. Discher et al.

    Tissue cells feel and respond to the stiffness of their substrate

    Science

    (2005)
  • Q.L. Erickson et al.

    Flash freezing of mohs micrographic surgery tissue can minimize freeze artifact and speed slide preparation

    Dermatol. Surg.

    (2011)
  • C.R. Ethier et al.

    Effects of latrunculin-B on outflow facility and trabecular meshwork structure in human eyes

    Investig. Ophthalmol. Vis. Sci.

    (2006)
  • R. Fuchshofer et al.

    The role of TGF-beta in the pathogenesis of primary open-angle glaucoma

    Cell Tissue Res.

    (2012)
  • Y.C. Fung

    Biomechanics

    (1993)
  • P.C. Georges et al.

    Cell type-specific response to growth on soft materials

    J. Appl. Physiol. (1985)

    (2005)
  • M.O. Gordon et al.

    The ocular hypertension treatment study: baseline factors that predict the onset of primary open-angle glaucoma

    Arch. Ophthalmol.

    (2002)
  • W.M. Grant

    Clinical measurements of aqueous outflow

    Am. J. Ophthalmol.

    (1951)
  • H. Han et al.

    Elasticity-dependent modulation of TGF-beta responses in human trabecular meshwork cells

    Investig. Ophthalmol. Vis. Sci.

    (2011)
  • M.J. Hoare et al.

    Cross-linked actin networks (CLANs) in the trabecular meshwork of the normal and glaucomatous human eye in situ

    Investig. Ophthalmol. Vis. Sci.

    (2009)
  • M. Honjo et al.

    Effects of rho-associated protein kinase inhibitor Y-27632 on intraocular pressure and outflow facility

    Investig. Ophthalmol. Vis. Sci.

    (2001)
  • J. Huang et al.

    Mechanical analysis of rat trabecular meshwork

    Soft Matter

    (2015)
  • M. Inatani et al.

    Transforming growth factor-beta 2 levels in aqueous humor of glaucomatous eyes

    Graefes Arch. Clin. Exp. Ophthalmol.

    (2001)
  • M. Johnson et al.

    Trabecular meshwork stiffness in the living human eye

    Investigative Ophthalmol. Vis. Sci.

    (2015)
  • Cited by (107)

    • Myocilin misfolding and glaucoma: A 20-year update

      2023, Progress in Retinal and Eye Research
    View all citing articles on Scopus
    View full text