252 - The anti-inflammatory effects of Portulaca oleracea L by partial suppression on NF-κB and MAPK activation and evaluation of its active constituents

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The purpose of the present study was to evaluate the anti-inflammatory effects of Portulaca oleracea L. (Purslane) extract on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and explore its potential mechanism. The RAW 264.7 cells were pretreated with Purslane extract for 4 h and subsequently induced by LPS for 12 h. It is of great concern that the bioactive compounds in Purslane extract and their metabolites in RAW 264.7 cell culture. What are the real bioactive compounds in the Purslaneextract that have the anti-inflammatory effects on RAW 264.7 cells, and when and how the bioactive compounds react? Based on this, 6 different groups were designed to be detected by LC/MS. (1): Purslane extract. (2): Blank medium without RAW 264.7 cells. (3): Medium pretreated by Purslane extract and induced by LPS without RAW 264.7 cells. (4): Blank medium in which RAW 264.7 cells were cultivated. (5): Medium induced by LPS in which RAW 264.7 cells were cultivated. (6): Medium pretreated by Purslane extract and induced by LPS in which RAW 264.7 cells were cultivated.

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Acknowledgments

This work was financially supported by Multi-Year Research Grant of University of Macau (MYRG2018-00169-ICMS) and Start-up Research Grant from University of Macau (SRG2015-00061-ICMS-QRCM).

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