Cytotoxicity of some Cameroonian spices and selected medicinal plant extracts
Graphical abstract
Effects of selected plant extracts (at 20 μg/ml) on the growth of blood capillaries on the chorioallantoic membrane of quail eggs. (A) DMSO (control): normal growth of blood capillaries on the CAM – no antiangiogenic effect; (B) Xylopia aethiopica; (C) Dorstenia psilirus; (D) Echinops giganteus; (E) Zinziber officinale. Extracts from D. psilirus and E. giganteus significantly inhibited angiogenesis in quail embryo.
Introduction
Pancreatic cancer is one of the most aggressive solid malignancies with high rate of resistance to most of the available treatment modalities, such as chemotherapy and radiotherapy (Wente et al., 2008). Only 20% of pancreatic cancers are amenable to surgical resection at presentation and despite the medical advances made over the last 20 years, pancreatic cancer appear to have benefited the least in terms of survival (Garcea et al., 2005). In the case of leukemia, MDR phenotypes have become a major concern to effective treatment. It is therefore important to develop new therapeutic strategies to treat malignancies. It was reported that excessive angiogenesis is an important factor of the pathogenesis of many industrialized western countries (Krenn and Paper, 2009). Therefore, compounds with anti-angiogenic properties are of importance in the treatment and prevention of malignancies as well as other chronic diseases (Paper, 1998, Carmeliet, 2003). Screenings of medicinal plants used as anticancer drugs have provided modern medicine with effective cytotoxic pharmaceuticals. More than 60% of the approved anticancer drugs in United State of America (from 1983 to1994) were from natural origin (Stévigny et al., 2005, Newman and Cragg, 2007). In the Cameroonian pharmacopoeia, there are still serious lacks of information on the use of large numbers of plants and spices traditionally employed in the treatment of several ailments, including cancers. It belongs to the scientific community to fill this gap. However, it has been recommended that ethnopharmacological usages such as immune and skin disorders, inflammatory, infectious, parasitic and viral diseases should be taken into account when selecting plants used to treat cancer, since these reflect disease states bearing relevance to cancer or cance-liker symptoms (Cordell et al., 1991, Popoca et al., 1998). Therefore, the present work was designed to investigate the cytotoxicity of some plants and spices used locally in Cameroon to treat cancers, but not inventoried in the national pharmacopoeia. The study was extended to the search of anti-angiogenic properties of some of the most active extracts.
Section snippets
Plant material and extraction
All medicinal plants used in the present work were collected in Dschang, western region of Cameroon, between January and February 2009. The spices were obtained from Dschang local market at the same period. The plants were identified at the National Herbarium (Yaounde, Cameroon) where voucher specimens were deposited under the references numbers (Table 1). The air-dried and powdered plant material was soaked in methanol for 48 h, at room temperature. The methanol extract was concentrated under
Results and discussion
In the US NCI plant screening program, a crude extract is generally considered to have in vitro cytotoxic activity, if the IC50 value following incubation between 48 and 72 h, is less than 20 μg/ml (Boik, 2001). In the present work, thirty-four selected plant extracts were tested (at 20 μg/ml) for their cytotoxic effects, and the results are summarized in Fig. 1, Fig. 2, Fig. 3 and Table 2. The results of Fig. 1, Fig. 2, Fig. 3 indicate that extracts from six of the 34 (17.6%) plants were able to
Acknowledgements
Authors are thankful to the National Herbarium of Cameroon (Yaounde). V.K. is also very grateful to the German Academic Exchange Service (DAAD) for the travel grant at the Johannes-Gutenberg University, Mainz, Germany.
References (119)
- et al.
Antimicrobial and cytotoxicity evaluation of Buchholzia coriacea stem bark
Fitoterapia
(2003) - et al.
Medicinal plants used by the Masango people in Gabon
Journal of Ethnopharmacology
(1994) - et al.
Antimicrobial and cytotoxic activities of the fruit essential oil of Xylopia aethiopica from Nigeria
Fitoterapia
(2004) - et al.
Bioactive crude plant seed extracts from the NCAUR oilseed repository
Phytomedicine
(2003) - et al.
Can ethnopharmacology contribute to development of new anti-cancer?
Journal of Ethnopharmacology
(1991) - et al.
Antihypertensive effects of Dorstenia psilurus extract in fructose-fed hyperinsulinemic, hypertensive rats
Phytomedicine
(2001) - et al.
Isothiocyanates, thiourees et thiocarbamates isolés de Pentadiplandra brazzeana
Phytochemistry
(1977) - et al.
Molecular prognostic markers in pancreatic cancer: a systematic review
European Journal of Cancer
(2005) - et al.
Chromatographic isolation of insecticidal amides from Piper guineense root
Journal of Chromatography A
(1992) - et al.
Investigation of in vitro anthelmintic activities of Pycnanthus angolensis and Sphenocentrum jollyanum
Fitoterapia
(2008)
Evaluation of the anthihepatotoxic activity of the biflavonoids of Garciaria kola seeds
Journal of Ethnopharmacology
Antioxidant and antibacterial activities of the leaf essential oils of Himalayan Lauraceae species
Food and Chemical Toxicology
Quantitative analysis of flavonols, flavones, and flavanones in fruits, vegetables and beverages by HPLC with photodiode array and mass spectrometric detection
Journal of Chromatography A
New amide alkaloid from the aerial part of Piper capense L.f. (Piperaceae)
Fitoterapia
Sterol glucosides from Prunella vulgaris
Phytochemistry
Inhibition of angiogenesis and inflammation by an extract of red clover (Trifolium pratense L.)
Phytomedecine
Antimicrobial activity of the methanolic extracts and compounds from Treculia obovoidea (Moraceae)
Journal of Ethnopharmacology
Antimicrobial activity of the methanolic extract and compounds from Teclea afzelii (Rutaceae)
South African Journal of Botany
Diospyrone, crassiflorone, and plumbagin. three antimycobacterial and anti-gonorrheal naphthoquinones from two Diospyros species
International Journal Antimicrobial Agents
Genotoxicities of nitropyrenes and their modulation by apigenin, tannic acid, ellagic acid and indole-3-carbinol in the Salmonella and CHO systems
Mutation Research
Kombic acid, a hydroquinone polyisoprenoic carboxylic acid from Pycnanthus kombo seed fat
Phytochemistry
In vitro cytotoxic activity of isolated acridones alkaloids from Zanthoxylum leprieurii Guill. et Perr
Bioorganic and Medicinal Chemistry
The structure of arundoin, cylindrin and fernenol triterpenoids of fernane and arborane groups of Imperata cylindrica var. Koenigii
Tetrahedron
Anticonvulsant activity of the volatile oil from the fruit of Tetrapleura tetraptera
Journal of Ethnopharmacology
Cycloartane derivatives from Garcinia lucida
Phytochemistry
Cytotoxic cycloartanes from Aglaia argentea
Phytochemistry
Cytotoxic activity of selected plants used as antitumorals in Mexican traditional medicine
Journal of Ethnopharmacology
Cyclodione, an unsymmetrical dimeric diterpene from Cylicodiscus gabunensis
Tetrahedron
Chemistry of the genus Dorstenia
Current Organic Chemistry
Antiplasmodial activity of lignans and extracts from Pycnanthus angolensis
Planta Medica
Traditional Medicine and Pharmacopoeia: Contribution to Ethnobotanical and Floristic Studies in Cameroon
Evaluation of aridanin, a glycoside, and aridan, an aqueous extract of Tetrapleura tetraptera fruit, on Schistosoma mansoni and S. bovis
Journal of Ethnopharmacology
In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extracts
Pharmaceutical Biology
Antibacterial effect of Zingiber officinale and Garcinia kola on respiratory tract pathogens
East African Medical Journal
Preliminary evaluation of the anti-inflammatory and anti-hepatotoxic activities of ‘Parsley’ Petroselinum crispum in rats
Journal of Natural Remedies
British Herbal Pharmacopoeia
Studies on some herbal drugs used against kidney stones in Saudi Folk medicine
Fitoterapia
Herbs that Heal Natural Remedies for Good Health
Inhibition of ultraviolet light-induced skin carcinogenesis in SKH-1 mice by apigenin, a plant flavonoid
Anticancer Research
Natural Compounds in Cancer Therapy
Feticheurs et medicines traditionnelles du Congo Brazzaville
Angiogenesis in health and disease
Nature Medicine
A new anti-inflammatory test, utilizing the chorioallantoic membrane of the chick embryo
British Journal of Pharmacology and Chemotherapy
The Useful Plants of West Tropical Africa
The Useful Plants of West Tropical Africa
Contribution to the knowledge of medicinal plants from Guinea-Bissau
Portugaliae Acta Biologica
In vitro antifungal activity of extract and plumbagin from the stem bark of Diospyros crassiflora Hiern (Ebenaceae)
Phytotherapy Research
Determination of chemical composition of Gnetum africanum (AFANG) seeds
Pakistan Journal of Nutrition
Vegetative Propagation of Shea, Kola And Pentadesma. Cocoa Research Institute, Ghana Annual Report (1987/88)
Novel antihyperglycemic terpenoid–quinones from Pycnanthus angolensis
Journal of Organic Chemistry
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