Cardiothoracic transplantation
Adenosine A2A receptor activation on CD4+ T lymphocytes and neutrophils attenuates lung ischemia–reperfusion injury

https://doi.org/10.1016/j.jtcvs.2009.08.033Get rights and content
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Objective

Adenosine A2A receptor activation potently attenuates lung ischemia–reperfusion injury. This study tests the hypothesis that adenosine A2A receptor activation attenuates ischemia–reperfusion injury by inhibiting CD4+ T cell activation and subsequent neutrophil infiltration.

Methods

An in vivo model of lung ischemia–reperfusion injury was used. C57BL/6 mice were assigned to either sham group (left thoracotomy) or 7 study groups that underwent ischemia–reperfusion (1 hour of left hilar occlusion plus 2 hours of reperfusion). ATL313, a selective adenosine A2A receptor agonist, was administered 5 minutes before reperfusion with or without antibody depletion of neutrophils or CD4+ T cells. After reperfusion, the following was measured: pulmonary function using an isolated, buffer-perfused lung system, T cell infiltration by immunohistochemistry, myeloperoxidase and proinflammatory cytokine/chemokine levels in bronchoalveolar lavage fluid, lung wet/dry weight, and microvascular permeability.

Results

ATL313 significantly improved pulmonary function and reduced edema and microvascular permeability after ischemia–reperfusion compared with control. Immunohistochemistry and myeloperoxidase content demonstrated significantly reduced infiltration of neutrophils and CD4+ T cells after ischemia–reperfusion in ATL313-treated mice. Although CD4+ T cell–depleted and neutrophil-depleted mice displayed significantly reduced lung injury, no additional protection occurred when ATL313 was administered to these mice. Expression of tumor necrosis factor-α, interleukin 17, KC, monocyte chemotactic protein-1, macrophage inflammatory protein-1, and RANTES were significantly reduced in neutrophil- and CD4+ T cell–depleted mice and reduced further by ATL313 only in neutrophil-depleted mice.

Conclusions

These results demonstrate that CD4+ T cells play a key role in mediating lung inflammation after ischemia–reperfusion. ATL313 likely exerts its protective effect largely through activation of adenosine A2A receptors on CD4+ T cells and neutrophils.

CTSNet classification

12
38.1

Abbreviations and Acronyms

A2AAR
adenosine A2A receptor
BAL
bronchoalveolar lavage
IgG
immunoglobulin G
IL-17
interleukin-17
IR
ischemia–reperfusion
LV
left ventricular
MCP
monocyte chemotactic protein
MIP
macrophage inflammatory protein
MPO
myeloperoxidase
RANTES
regulated on activation, normal T expressed and secreted
TNF-α
tumor necrosis factor-alpha
WBC
white blood cell

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Disclosures: Drs Linden and Kron were shareholders in Adenosine Therapeutics, LLC, the corporation that provided ATL313, during the time of this study.

This study was funded by NIH/NHLBI grants RO1 HL077301 (to V.E.L.) and PO1 HL073361 (to J.L.).