Original ArticleCholesterol succinyl chitosan anchored liposomes: preparation, characterization, physical stability, and drug release behavior
Section snippets
Materials
Biomedical grade chitosan (deacetylation degree was 92%, viscosity average molecular weight was 8.0 × 104 Da) was supplied by Yuhuan Ocean Biochemical Co., Ltd. (Zhejiang, China). Three CHCS conjugates were synthesized by the method that we previously reported.14 The substitution degrees (DS) of the cholesterol moiety of CHCS, defined as the amount of cholesterol moieties per 100 glucosamine units of chitosan, was determined by the colloid titration method,15 and the DS values of three CHCS
Preparation and characterization of CALs
In our study, the different polysaccharide/lipid weight ratios were investigated to prepare CAL. Figure 1 shows the effects of polysaccharide/lipid weight ratio on the size (Figure 1, A) and the zeta potential (Figure 1, B) of CAL, and the same trend occurred among CALs with different DS of the cholesterol moiety. Compared with the plain liposomes (size 148.2 ± 3.0 nm and zeta potential −4.75 mV), CALs had significantly larger sizes and positive zeta potentials, which indicated that CHCS
Discussion
Recently, many investigations into CCL have been performed to enhance the stability of liposomes and increase their intestinal uptake.22, 23, 24 We previously hoped to use CCL as the carrier of anticancer drugs, but the chitosan layer formed by the electrostatic attractive interaction between the positively charged amino groups in chitosan molecules and the negatively charged surface of liposomes was not effective enough to significantly stabilize liposomes in vitro, so that the anchoring
Acknowledgment
The authors thank Drs. Xindu Yang, Wenzhi Yang, and Hongli Chen for their helpful discussion and suggestions.
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This work was supported by the National Natural Science Foundation of China (grant no. 30900303).