Elsevier

Carbohydrate Research

Volume 18, Issue 3, July 1971, Pages 381-388
Carbohydrate Research

Nojirimycin and d-glucono-1,5-lactone as inhibitors of carbohydrases

https://doi.org/10.1016/S0008-6215(00)80274-8Get rights and content

Abstract

Nojirimycin and d-glucono-1,5-lactone are powerful inhibitors of glucosidases, but poor inhibitors of exo-glucanases, endo-glucanases, and related enzymes. Nojirimycin can be used to differentiate between α-glucosidase and exo-α-d-glucanase, and between β-glucosidase and exo-β-d-glucanase. The structural similarity of the two inhibitors is discussed in relation to the mechanism of action of the enzyme types. Some data on d-glucal are included.

References (27)

  • S. Inouye et al.

    Tetrahedron

    (1968)
  • Y.C. Lee

    Biochem. Biophys. Res. Commun.

    (1969)
  • D.H. Leaback

    Biochem. Biophys. Res. Commun.

    (1968)
  • H. Paulsen et al.

    Advan. Carbohyd. Chem.

    (1968)
  • T. Niwa et al.

    Agr. Biol. Chem.

    (1970)
  • E.T. Reese et al.

    Can, J. Biochem.

    (1968)
  • E.T. Reese et al.

    Dev. Ind. Microbiol.

    (1960)
  • J. Conchie et al.

    Biochem. J.

    (1957)
  • J. Conchie et al.

    Biochem. J.

    (1967)
  • J. Conchie et al.

    Biochem. J.

    (1957)
  • G.A. Levvy et al.

    Biochem. J.

    (1964)
  • M.L. Hackert et al.

    Chem. Commun.

    (1969)
  • W.P. Jencks
  • Cited by (168)

    • The promising application of a β-glucosidase inhibitor in the postharvest management of Volvariella volvacea

      2022, Postharvest Biology and Technology
      Citation Excerpt :

      The control treatment was carried out using 257 mL H2O solution. D-glucono-1,5-lactone (DGase) (MacklinCatalogue No: D810428), usually used as a coagulant of the traditional Chinese food tofu, is also a potent inhibitor of β-glucosidase (Deshpande et al., 1984; Reese et al., 1971). It was added to H2O to prepare a 300 μM DGase solution.

    • Influence of cellulase gene expression and cellulolytic activity on cellulose utilization in different Volvariella volvacea strains

      2020, Scientia Horticulturae
      Citation Excerpt :

      Besides, some nonionic surfactants can increase the yields of the enzymes cellulases, amylase, and xylanase in fungal cultures (Reese and Maguire, 1969). Nojirimycin and d-glucono-1,5-lactone are powerful inhibitors of glucosidases, but poor inhibitors of exo glucanases, endo-glucanases (Reese et al., 1971). The above studies have reminded us that the use of suitable inducers and the removal of inhibitors may greatly promote cellulase activity, thereby increasing the yield of V. volvacea.

    • Biochemical and structural analyses of a bacterial endo-β-1,2-glucanase reveal a new glycoside hydrolase family

      2017, Journal of Biological Chemistry
      Citation Excerpt :

      During the purification from the cell extract of C. arvensicola, β-1,2-glucan-hydrolyzing activity was defined as the amount of released reducing ends (Glc equivalence) in ∼24 h based on a Glc standard curve. Endo-β-1,2-glucanase activity was analyzed by TLC in the presence of GDL, a known β-glucosidase inhibitor (42). The enzymatic reaction was carried out in the reaction mixture described above in the presence of 50 mm GDL, and an aliquot of the reaction mixture was analyzed by TLC.

    View all citing articles on Scopus
    View full text