Antioxidant activity of thioureidic derivatives I
Introduction
Several studies have investigated the in vitro biological activity of synthetic thioureidic derivatives and have established important structure–activity relationships. Thus, we have investigated the free-radical scavenging and antioxidant effectiveness of synthetic thioureidic derivatives, which demonstrated antiamebic activity, by using different in vitro methods:
- 1
in aqueous phase, detection of the relative antioxidant capability to scavenge the chromogenic radical cation 2,2′-azinobis(3-ethyl-benzothiazoline-6-sulfonate (ABTS+) in comparison with Trolox and expressed as TEAC [1], [2]. ABTS+, cation radical, is a blue–green cromogen with a characteristic absorption at 734 nm. The antioxidant activities of compounds are expressed as TEAC, trolox equivalent antioxidant capacity;
- 2
heat-induced oxidation of an acqueous emulsion system of β-carotene and linoleic acid was measured by the method described by Pratt (1992) [3];
- 3
superoxide anion enzymatic generation assay. Superoxide anion was generated in an enzymatic system by preparing a mixture of xanthine and xanthine oxidase [4];
- 4
xanthine oxidation inhibition assay; in this assay xanthine oxidase activity was evaluated by the spectrophotometric measurement of the formation of uric acid by xanthine [4].
Section snippets
Chemicals
ABTS (2,2′-azinobis-3-ethilbenzothiazoline-6 sulfonic acid), Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), potassium persulfate, linoleic acid, Tween 20, butylhydroxytoluene, β-carotene, EDTA, bovine serum albumin (BSA), nitroblue–tetrazolium (NBT), xanthine, xanthine oxidase (XOD), sodium carbonate, sodium phosphate monobasic and sodium phosphate dibasic were obtained by Sigma Aldrich (Gillingham, Dorset, UK). The solvents were obtained by Carlo Erba reagent. Nanopure water
Free Radical scavenging assay
Trolox (a water soluble vitamin E analogue) equivalent antioxidant capacity (TEAC) has been used to determine the radical scavenging abilities of synthetic thioureidic derivatives as electron or H donating agents throughout their ability to scavenge ABTS+ The TEAC value resulting for compounds 1–6 are summarized in Table 1.
Linoleic acid autoxidation assay
The antioxidant effect of synthetic thioureidic derivatives was also measured by autoxidation of β-carotene assay. The value of antioxidant activity (AA) measured a t=60 and
Discussion
A fascinating hypothesis raised in the past few years is that the health-promoting action of some foods could be due to the presence of nonessential components, such as polyphenols (many with antioxidant potential) that could contribute to the modulation of the oxidative balance in vivo
On the other hand reactive oxygen species (ROS) are considered related to many diseases, including atherosclerosis, liver injury, aging, inflammation, neurovegetative diseases, and cancer.
The present study deals
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