RESEARCH NOTE
Developmental expression of a Fasciola hepatica sequence homologous to ABC transporters1

https://doi.org/10.1016/S0020-7519(98)00102-7Get rights and content

Abstract

Polymerase chain reaction and cDNA library screening approaches were employed to identify a putative member of the highly conserved family of ATP-binding cassette transport proteins from Fasciola hepatica. At the predicted protein level, the F. hepatica sequence identified in the present study shares 43% and 36% identity with the Schistosoma mansoni SMDR2 and human MDR1 ATP-binding cassette transport sequences, respectively. Northern blot and reverse transcriptase–PCR analyses have demonstrated that expression of the F. hepatica ABC-transporter homologue is confined to immature parasites. The biochemical basis for the stage-specific expression of the ATP-binding cassette transporter homologue within F. hepatica remains to be determined.

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Acknowledgements

This work was supported by CIBA Animal Health Limited (Australia), Agriculture Victoria and Monash University. M.B.R. was supported by the Department of Agriculture “Nancy Millis” Postgraduate Scholarship.

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1

Note:The sequence data reported in this paper have been submitted to GenBank and assigned the accession numbers L36247 and L36248.

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