Journal of Biological Chemistry
Volume 244, Issue 17, 10 September 1969, Pages 4664-4667
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CHEMISTRY AND METABOLISM OF MACROMOLECULES
Preparation and Properties of α- and β-Chains from Human Hemoglobin

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A new procedure is described using carboxymethyl cellulose and diethylaminoethyl cellulose columns for the preparation of native α and β chains from human hemoglobin. The chains appeared to be more stable than those obtained by earlier methods and permitted the preparation of modified chains containing meso- and deuterohemes. The kinetics of carbon monoxide binding was determined for proto-, meso-, and deutero-α and -β chains by both stopped flow and flash photolysis methods. The kinetic properties of β—SH chains with protoheme are similar to those reported for hemoglobin H, and their rate of reaction with carbon monoxide is more than twice as great as that for α—SH chains.

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