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Dynamic packing of ion-exchange chromatographic columns

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References (4)

  • O. Mikes
    (1966)
  • C.D. Scott et al.

    Proc. Soc. Exptl. Biol. Med.

    (1967)
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Cited by (55)

  • Segmented flow and curtain flow chromatography: Overcoming the wall effect and heterogeneous bed structures

    2014, Journal of Chromatography A
    Citation Excerpt :

    It is difficult to define the exact point in time at which modern HPLC was established since along with the application of pressure packing techniques came a decrease in the size of particles that could be employed for these more efficient modern columns. To our reckoning, however, we believe that the birth appears to originate from the works of Scott and Lee [1] in 1969, where they packed columns using 10 μm ion exchange media in a downward slurry high pressure system. These early high pressure slurry packing methods were a development from the ‘tap and fill’ dry packing methods of earlier days where larger particle sizes in the order of tens of microns or more were employed.

  • Enhancing the separation performance of the first-generation silica monolith using active flow technology: Parallel segmented flow mode of operation

    2014, Journal of Chromatography A
    Citation Excerpt :

    Active flow technology (AFT) is the term that encompasses a range of new chromatography columns that are designed to eliminate wall effects, minimise solute band broadening due to radial heterogeneity [1–6] and decrease the peak volume of eluting components.

  • Improving the performance of narrow-bore HPLC columns using active flow technology

    2014, Microchemical Journal
    Citation Excerpt :

    However, a main drawback in decreasing the column internal diameter to achieve such an advantage is that packing columns with the same quality as 4.6 mm i.d. become difficult since the contribution made by frictional forces at the wall during the packing process form a greater proportion of the bed geometry compared to 4.6 mm i.d. columns. Thus, the radial heterogeneity of the packed bed increases, as the detrimental wall effect becomes more prominent [1–6], since the area of the wall in relation to the cross sectional surface area of the column is far greater than compared to the analytical scale 4.6 and 3.0 mm i.d. columns. Furthermore, greater care must be paid to minimize the extra column dead volume effects since the peak volume at elution is small for narrow-bore columns.

  • Colloidal aspects and packing behaviour of charged microparticulates in high efficiency ion chromatography

    2012, Journal of Chromatography A
    Citation Excerpt :

    Unfortunately, most of these options are not applicable to IC phases due to solvent compatibility. Both classic [18] and recent works [19] discussing the packing of ion exchange particles do not divulge all the slurry packing conditions. The rheology of the suspension comes into play when the slurry is driven from the slurry reservoir into the empty column blank (Supplementary Fig. S1).

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Research supported by the National Institute of General Medical Sciences and the U.S. Atomic Energy Commission.

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