An improved method for thin-layer chromatography of nucleotide mixtures containing32P-labeled orthophosphate
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2017, Analytical BiochemistryCitation Excerpt :A thorough mechanistic understanding of such ATP/GTP-driven processes requires a reliable means to quantify nucleotide hydrolysis under dynamic conditions in complex, multi-component media. Many assays have been developed to measure ATP/GTP hydrolysis, which focus on monitoring the amount of a) ADP/GDP formed [4,5] or b) inorganic phosphate (Pi) released [6,7] during the course of a reaction. In the case of the former, assays typically require separation of nucleoside diphosphate (NDP) species formed from unused NTP species by high-performance liquid chromatography (HPLC) [5].
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National Institute of Neurological Disease and Stroke, National Institutes of Health, Public Health Service, U.S. Department of Health, Education and Welfare, Bethesda, Md. 20014 U.S.A.