An improved method for thin-layer chromatography of nucleotide mixtures containing32P-labeled orthophosphate

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Abstract

Development of poly(ethylene)imine cellulose thin layers with phosphate solutions gives improved resolution of complex mixtures of nucleotides. Phosphate development also minimizes the tailing of highly radioactive orthophosphate present in the mixtures and thus facilitates chromatographic analysis of crude acid extracts of phosphate-labeled bacteria. Conditions employing phosphate development are described which give semi-quantitative resolution of the ribonucleoside triphosphate components of such extracts after one-dimensional chromatography as well as two-dimensional systems for quantitative resolution of the major nucleotide components.

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National Institute of Neurological Disease and Stroke, National Institutes of Health, Public Health Service, U.S. Department of Health, Education and Welfare, Bethesda, Md. 20014 U.S.A.

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