Use of gel filtration in the assay of urinary enzymes

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Abstract

Gel filtration of urine provides a simple and rapid, yet reliable, method for the separation of lactate dehydrogenase, alkaline and acid phosphatase, leucine amino-peptidase, arylsulphatase, and β-glucuronidase from interfering substances. It removes inhibitors more completely than dialysis and separates all spurious lactate dehydrogenase and alkaline phosphatase activities from the protein enzymes.

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    Present address: Dr. M. Werner, Division of Clinical Pathology and Laboratory Medicine, U.C. Medical Center, San Francisco, Calif. 94122 (U.S.A.).

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