Antinuclear Antibodies: Diagnostic Markers for Autoimmune Diseases and Probes for Cell Biology

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Early studies pointed to the directions for subsequent investigations and there has been continuing identification of other circulating autoantibodies, characterization of their respective cellular antigens, and demonstration of the relationship between autoantibodies and clinical syndromes. The antibodies identified in these studies have been used extensively by investigators in molecular and cell biology as powerful probes for understanding the precursor messenger RNA (mRNA) splicing. Autoantibodies designated SS-A or Ro and SS-B or La were identified in patients with Sjogren's syndrome (SS) and SLE, and have been shown to target intracellular proteins that may be involved with regulation of RNA polymerase III function. Investigators in the field had long perceived that these spontaneously occurring auto-antibodies in human disease would turn out to be useful reagents in cell biology. It was also appreciated that it was necessary to characterize the molecular nature of the auto-antigens so that in addition to the immunochemical properties, their structure and function might be determined. One of the purposes of this chapter is to show that the new molecular biology of cellular antigens and auto-antibodies could now be providing insights into comprehending some features of autoimmunity. In addition, there appears to be a need for the synthesis of the wealth of information that has accumulated and an evaluation of what it might signify.

References (232)

  • I. Bozzoni et al.

    Splicing of Xenopus laevis ribosomal protein RNAs is inhibited in vivo by antisera to snRNP.

    J. Mol. Biol.

    (1984)
  • T. Mimori et al.

    Autoantibodies to the U2 small nuclear ribonucleoprotein in a patient with sclerodermapolymyositis overlap syndrome.

    J. Biol. Chem.

    (1984)
  • H.L. Franco et al.

    Autoantibodies directed against sicca syndrome antigens in the neonatal lupus syndrome.

    J. Am. Acad. Dermatol.

    (1981)
  • T. Mimori et al.

    Characterization of the DNA-binding protein Ku recognized by autoantibodies from patients with rheumatic disorders.

    J. Biol. Chem.

    (1986)
  • P.H. Schur et al.

    Precipitating antibodies to ribosomes in the serum of patients with systemic lupus erythematosus.

    Immunochemistry

    (1967)
  • M.M. Hargraves et al.

    Presentation of two bone marrow elements: The “tart” cells and the “L.E.” cell.

    Mayo Clin. Proc.

    (1948)
  • W.C. Robbins et al.

    Complement fixation with cell nuclei and DNA in lupus erythematosus.

    Proc. Soc. Exp. Biol. Med.

    (1957)
  • R. Holman et al.

    The reaction of the lupus erythematosus (L.E.) cell factor with deoxyribonucleoprotein of the cell nucleus.

    J. Clin. Invest.

    (1959)
  • H.G. Kunkel et al.

    Ciba Found. Symp. Cell. Aspects Immun., Basel

    “Multiple “Autoantibodies” to cell constituents in systemic lupus erythematosus.”

    (1960)
  • E.M. Tan et al.

    Characteristics of a soluble nuclear antigen precipitating with sera of patients with systemic lupus erythematosus.

    J. Immunol.

    (1966)
  • J.D. Northway et al.

    “Differentiation of antinuclear antibodies giving speckled staining patterns in immunofluorescence.” Clin. Immunol. Immunopathol.

    (1972)
  • M. Mattioli et al.

    Physical association of two nuclear antigens and mutual occurrence of their antibodies: The relationship of the Sm and RNA protein (Mo) systems in SLE sera.

    J. Immunol.

    (1973)
  • M.R. Lerner et al.

    Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.

    Proc. Natl. Acad. Sci. U.S.A.

    (1979)
  • H. Busch et al.

    SnRNAs, SnRNPs and RNA processing.

    Annu. Rev. Biochem.

    (1982)
  • C. Brunel et al.

    snRNPs and scRNPs in eukaryotic cells.

    Prog. Mol. Subcell. Biol.

    (1985)
  • M. Mattioli et al.

    Heterogeneity of RNA protein antigens reactive with sera of patients with systemic lupus erythematosus.

    Arthritis Rheum.

    (1974)
  • M.A. Alspaugh et al.

    Antibodies to cellular antigens in Sjögren's syndrome.

    J. Clin. Invest.

    (1975)
  • M.R. Lerner et al.

    Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus.

    Science

    (1981)
  • J.H. Shero et al.

    Autoantibodies to topoisomerase I are found in sera from scleroderma patients.

    Science

    (1986)
  • G.G. Maul et al.

    Topoisomerase I identified by scleroderma 70 antisera: Enrichment of topoisomerase I at the centromere in mouse mitotic cells before anaphase.

    Proc. Natl. Acad. Sci. U.S.A.

    (1986)
  • H.H. Guldner et al.

    Scl-70 autoantibodies from scleroderma patients recognize a 95KD protein identified as DNA topoisomerase I.

    Chromosoma

    (1986)
  • K. Miyachi et al.

    Autoantibody to a nuclear antigen in proliferating cells.

    J. Immunol.

    (1978)
  • R. Bravo et al.

    Cyclin/PCNA is the auxiliary protein of DNA polymerase delta.

    Nature (London)

    (1987)
  • G. Prelich et al.

    Functional identity of proliferating cell nuclear antigen and a DNA polymerase delta auxiliary protein.

    Nature (London)

    (1987)
  • V.W. Yang et al.

    A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.

    Proc. Natl. Acad. Sci. U.S.A.

    (1981)
  • E.M. Tan et al.

    DNA and antibody to DNA in the serum of patients with systemic lupus erythematosus.

    J. Clin. Invest.

    (1966)
  • B.D. Stollar

    Antibodies to DNA.

    CRC Crit. Rev. Biochem.

    (1986)
  • B.D. Stollar et al.

    The antigenic determinants of denatured DNA reactive with lupus erythematosus serum.

    Proc. Natl. Acad. Sci. U.S.A.

    (1962)
  • M.J. Fritzler et al.

    Antibodies to histones in drug-induced and idiopathic lupus erythematosus.

    J. Clin. Invest.

    (1978)
  • R.L. Rubin et al.

    Specificity of anti-histone antibodies in systemic lupus erythematosus.

    Arthritis Rheum.

    (1982)
  • G.E. Conner et al.

    Protein antigens of the RNA-protein complexes detected by anti-Sm and anti-RNP antibodies found in serum of patients with systemic lupus erythematosus and related disorders.

    J. Exp. Med.

    (1982)
  • D.E. Fisher et al.

    Synthesis and assembly of human small nuclear ribonucleoproteins generated by cell-free translation.

    Proc. Natl. Acad. Sci. U.S.A.

    (1983)
  • H. Yamagata et al.

    Molecular properties of the Ro/SSA antigen and enzyme-linked immunosorbent assay for quantitation of antibody.

    J. Clin. Invest.

    (1984)
  • C.E. Harmon et al.

    The importance of tissue substrate in the SS-A/Ro antigen-antibody system.

    Arthritis Rheum.

    (1984)
  • T.-S. Lieu et al.

    Identification of the SS-A/Ro intracellular antigen with autoimmune sera.

    J. Immunol. Methods

    (1984)
  • A.M. Francoeur et al.

    Interaction between VA-RNA and the lupus antigen La: Formation of a ribonucleoprotein particle

    in vitro. Proc. Natl. Acad. Sci. U.S.A.

    (1982)
  • E.K.L. Chan et al.

    Epitopes, structural domains and asymmetry of amino acid residues in SS-B/La nuclear protein.

    J. Immunol.

    (1986)
  • E.K.L. Chan et al.

    The small nuclear ribonucleoprotein SS-B/La binds RNA with a conserved protease-resistant domain of 28 KD Mol.

    Cell Biol.

    (1987)
  • T. Mimori et al.

    Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies in sera from patients with polymyositis-scleroderma overlap.

    J. Clin. Invest.

    (1981)
  • A.M. Francoeur et al.

    Identification of Ki (Ku, p70/p80) autoantigens and analysis of anti-Ki autoantibody reactivity.

    J. Immunol.

    (1986)
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