[56] Quantitative determination of carotenoids in photosynthetic tissues
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Purple bacteria screening for photoautohydrogenotrophic food production: Are new H<inf>2</inf>-fed isolates faster and nutritionally better than photoheterotrophically obtained reference species?
2022, New BiotechnologyCitation Excerpt :The quality of the protein is classified according to the EAAI as superior (>1), high (0.95–1), good (0.86–0.95), useful (0.75–0.86) or inadequate (<0.75) [37]. Lastly, bacteriochlorophyll a (bchl a) and total carotenoid content was determined by an acetone/methanol solvent (7:2 v/v) extraction, followed by spectrophotometric analysis and conversion with the Lambert-Beer law [38,39] as described in [13]. A standard deviation (SD) is given for the maximum growth rates and the lag phases, which is the SD on the fit of the Gompertz model (95% confidence).
Two-step strategy for obtaining dunaliella sp. biomass and β-carotene from anaerobically digested poultry litter wastewater
2019, International Biodeterioration and BiodegradationCitation Excerpt :The microalgae samples were filtered with a 0.22-μm-pore-size glass fiber filter (GTY1-BLQW Φ100 mm/0.22, Midwest Group, China) for dry cell weight measurement according to a published method (Lee and Shen, 2004). The β-carotene amount was measured spectrophotometrically at 455 nm and calculated according to Jensen's equation (Liaaen-Jensen and Jensen, 1971). First, 2–5 mL (VD, mL) of sample was removed from the flask and centrifuged at 8000 rpm for 5 min.
Rapid detection of haloarchaeal carotenoids via liquid-liquid microextraction enabled direct TLC MALDI-MS
2013, TalantaCitation Excerpt :The extracts from each treatment was equalized using acetone and the absorption spectrum was obtained from 350–600 nm in a Lambda 950, spectrophotometer (Perkinelmer Inc., USA). The total carotenoid pigments quantified from each extract were determined using standard calculation for carotenoid pigments involving an average extinction coefficient [21]. The carotenoid pigments extracted from H. mediterranei cells treated with TiO2 NPs were separated by thin layer chromatography (TLC) plates pre-coated with silica gel (Merck 5553, EMerk Inc., Germany) using 50% acetone in n-heptane (v/v) as a developing solvent [12].
Solanum nigrum L. antioxidant defence system isozymes are regulated transcriptionally and posttranslationally in Cd-induced stress
2011, Environmental and Experimental BotanyCitation Excerpt :For the extraction of photosynthetic pigments leaf samples (L5 and L7) were grinded in 80% acetone with quartz sand using a pre-chilled mortar and pestle. The extracts were centrifuged at 2 200 × g for 15 min and the contents of chlorophylls and carotenoids were quantified according to Strain et al. (1971) and Liaaen-Jensen and Jensen (1971), respectively. Determination of thiobarbituric acid reacting substances (TBARS) as an indicator of membrane lipid peroxidation in leaves and roots was measured in terms of malondialdehyde (MDA) content, according to Heath and Packer (1968).
Photosynthetic bacterial growth and productivity under continuous illumination or diurnal cycles with olive mill wastewater as feedstock
2010, International Journal of Hydrogen EnergyCitation Excerpt :At the end of each experiment, the cell biomass was separated from the processed OMW by centrifugation at 10,000 × g for 15 min at 4 °C. The pellet of the centrifugation process was analyzed for PHB and carotenoid content, by following the method of Bowker (1981) [18] and acetone extraction by Liaaen-Jensen and Jensen (1971) [19], respectively. Total carbonate/bicarbonate amount was detected analytically from the supernatant of the centrifuged culture, at the end of each experiment by following the titration procedure that was given previously [20].
Tepidamorphus gemmatus gen. nov., sp. nov., a slightly thermophilic member of the Alphaproteobacteria
2010, Systematic and Applied Microbiology