Chapter 12 In Situ Hybridization
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Cited by (32)
Mechanical force induced DNA double-strand breaks: Ultrasound
2022, EnzymesCitation Excerpt :Currently, several methodologies can be used to detect DSBs both in vivo and in vitro [14–21], such as polymerase chain reaction, immunological assays, in situ hybridization, etc. Despite the availability of these methods, it is difficult to evaluate the number of double-strand breaks in a quantitative manner, especially for long genome-sized DNA molecules [22–24]. Recently, it has been demonstrated that the direct visualization of single giant DNA molecules using fluorescence microscopy can provide useful information on the structure and function of genomic DNA molecules in solution [25–28], and can be used to analyze DSBs in a quantitative manner.
Global optimization using Gaussian processes to estimate biological parameters from image data
2019, Journal of Theoretical BiologyCitation Excerpt :An example of such data is ISH data. ISH is a technique in which a complementary strand to the nucleic acid of interest, with a reporter molecule attached, is applied to the tissue of interest (Hargrave et al., 2006; McFadden, 1995). Upon addition of a substrate, a chemical reaction catalysed by the reporter molecule takes place which reveals the location of a specific segment of nucleic acid, for instance DNA or RNA.
Protective effect of ascorbic acid against double-strand breaks in giant DNA: Marked differences among the damage induced by photo-irradiation, gamma-rays and ultrasound
2015, Chemical Physics LettersCitation Excerpt :Immunological assays are also commonly used for the detection of oxidative DNA damage through the use of an antibody or immunoglobulin [7,9]. In situ hybridization provides information on specific changes in certain DNA sequences [7,10]. A comet assay can detect double-strand breaks in DNA, although a quantitative evaluation is almost impossible [7,11].
Presence of prolactin mRNA in extra-pituitary brain areas in the domestic turkey
2012, Acta HistochemicaCitation Excerpt :No labeling on brain sections was observed with the sense cRNA probes (data not shown). The criteria used in the present study have been accepted as sufficient verification of the specificity for probes utilized for in situ hybridization (Young, 1990; Wilkinson, 1992; McFadden, 1995; Chesselet et al., 1996). Data were organized by coronal brain sections, which were taken from approximately 1.0 mm rostral to the optic chiasma to approximately 1.0 mm caudal to the median eminence and pituitary.