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A novel member of the NK-lysin protein family is developmentally regulated and secreted by Fasciola hepatica

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Acknowledgements

This work was supported by CIBA Animal Health Limited (Australia) and Agriculture Victoria. MBR was supported by the Department of Agriculture ‘Nancy Millis’ Postgraduate Scholarship.

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References (42)

Cited by (25)

  • Saposin-like proteins in Opisthorchis felineus and related opisthorchids

    2020, Infection, Genetics and Evolution
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    Three transcripts coding for paralogous SAPs were also identified in another large liver fluke Fasciola hepatica: FhSAP-1, FhSAP-2 and FhSAP-3. The corresponding proteins were found in ESP of adult parasites, but only FhSAP-1 was specific for immature worms (Reed et al., 2000; Di Maggio et al., 2016). In functional tests, FhSAP-2 showed lytic activity on human blood cells (Espino and Hillyer, 2003).

  • Characterization and vaccine potential of Fasciola gigantica saposin-like protein 1 (SAP-1)

    2017, Veterinary Parasitology
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    This suggests that the SAPs are involved in the lysis of host cells for further enzymatic processing and uptake by the parasites. Two SAPs encoding cDNAs from F. hepatica (FhSAP-1, FhSAP-2), and three SAPs encoding cDNA from F. gigantica (FgSAP-1, FgSAP-2, FgSAP-3) were reported (Reed et al., 2000; Espino and Hillyer, 2003; Grams et al., 2001; Cabán-Hernández and Espino, 2013). FgSAP-1 is expressed at NEJ and early stage of F. gigantica, whereas FgSAP-2 and FgSAP-3 are expressed in late juveniles to adult stages but not at metacercaria, NEJ and early juvenile stage (Grams et al., 2001).

  • Adjuvant-enhanced antibody and cellular responses to inclusion bodies expressing FhSAP2 correlates with protection of mice to Fasciola hepatica

    2016, Experimental Parasitology
    Citation Excerpt :

    Fasciola species use these lytic proteins to cause lysis of the hosts' erythrocytes and leuckocytes so that their contents can be digested further for the parasite's nourishment (Espino and Hillyer, 2003). In F. hepatica, there are two isoforms (SAP1 and SAP2) (Espino and Hillyer, 2003; Reed et al., 2000). SAP1 is expressed in immature and adult stages (Reed et al., 2000), whereas SAP2 is highly expressed in newly excysted juvenile, 3-week juvenile up to adult stages and also in non-embryonated eggs (Caban-Hernandez and Espino, 2013; Espino and Hillyer, 2003).

  • Differential expression and localization of saposin-like protein 2 of Fasciola hepatica

    2013, Acta Tropica
    Citation Excerpt :

    If FhSAP2 has an amoebapore-like capacity and forms ion channels within lipid bilayer membranes, it may contribute to the lymphotoxic activities attributed to F. hepatica excretory–secretory material (Cervi et al., 2009; Goose, 1978). Reed et al. also suggested a role for FhSAP1 in lipid metabolism, another SAPLIP in F. hepatica, since insufficiency in de novo fatty acid and cholesterol synthesis is a trait of trematodes (Reed et al., 2000). Also, we cannot discount a possible role in parasite tegument formation, as seen for other trematode tegumental proteins (Tran et al., 2010).

  • The saposin-like proteins 1, 2, and 3 of Fasciola gigantica

    2006, Molecular and Biochemical Parasitology
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Note: Nucleotide sequences data reported in this paper are available in the GenBank database under the accession number U58467.

1

Present address: The Walter and Eliza Hall Institute of Medical Research, Royal Pde., Parkville, Victoria 3052, Australia.

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