Brief communication
Studies on the anticlastogenic effect of turmeric and curcumin on cyclophosphamide and mitomycin C In Vivo

https://doi.org/10.1016/S0278-6915(97)81791-7Get rights and content

Abstract

Turmeric and its main constituent curcumin were assessed in vivo for their anticlastogenic potential. In one experimental set, Swiss albino male mice were given turmeric (8, 12 and 16 mg/kg body weight) or curcumin (2, 4 and 8 mg/kg body weight) as a single intraperitoneal injection. In another set, the mice were given 8 mg/kg body weight of turmeric or one of three concentrations of curcumin (2, 4 and 8 mg/kg body weight) as a dietary supplement by gavage for 7 consecutive days. 30 min after the last dose the mice were administered a single acute dose of two known clastogens, cyclophosphamide (CP) (20 mg/kg body weight) or mitomycin C (MMC) (1.5 mg/kg body weight). After 18 hr, chromosome preparations were made from bone marrow cells. The endpoints studied were chromosome aberrations and damaged cells. Clastogenicity of the chemicals was compared using turmeric- or curcuminprimed and non-primed animals. As single agents turmeric and curcumin were not clastogenic even after 7 days of priming. Turmeric/curcumin could not inhibit CP- or MMC-induced clastogenicity. Although curcumin is reported to be the active chemopreventive principle in turmeric effective against a number of potential carcinogens in several experimental systems, it was virtually ineffective against the clastogenicity of CP or MMC at the doses tested.

References (41)

  • S. Abraham et al.

    Mutagenic potential of the condiments, ginger and turmeric

    Cytologia

    (1976)
  • W. Au et al.

    Studies on clastogenic effects of biological stain and dyes

    Environmental Mutagenesis

    (1979)
  • M.A. Azuine et al.

    Chemopreventive effect of turmeric against stomach and skin tumours induced by chemical carcinogens in Swiss mice

    Nutrition and Cancer

    (1992)
  • N.R. Bachur et al.

    NADPH-cytochrome P450 reductase activation of quinone anti-cancer agents to free radicals

  • A.M. Bonin et al.

    Mutagenicity testing of some approved food additives with the Salmonella/microsome assay

    Food Technology

    (1980)
  • R.N. Chopra et al.

    Glossary of Indian Plants

    (1956)
  • T. Das et al.

    Modification of clastogenicity of three known clastogens by garlic extract in mice in vivo

    Environmental and Molecular Mutagenesis

    (1993)
  • G.R. Douglas et al.

    Genotoxicity tests as predictors of carcinogens: an analysis

    Mutation Research

    (1988)
  • B.D. Duncan

    Multiple range test

    Biometrics

    (1955)
  • A.K. Giri et al.

    Effects of vitamin C and vitamin A on post-chromosomal aberration in vivo induced by metanil yellow and zinc chloride

    Cytologia

    (1988)
  • Cited by (32)

    • Validation of the 3D Skin Comet assay using full thickness skin models: Transferability and reproducibility

      2018, Mutation Research - Genetic Toxicology and Environmental Mutagenesis
    • Ameliorative effect of two Ayurvedic herbs on experimentally induced arsenic toxicity in calves

      2017, Journal of Ethnopharmacology
      Citation Excerpt :

      and dried root powder of ginger (Z. officinale Rosc.) are used widely by Indian population as food additives (Mukhopadhyay et al., 1998; Thapliyal et al., 2002). Both of these plant origin materials are used extensively in Ayurvedic system of medicine for their anti-inflammatory (Chandra and Gupta, 1972), antimicrobial (Lutomski et al., 1974), hepatoprotective (Subramanian and Selvam, 1999) anticancer (Thapliyal et al., 2002), antioxidant (Jagetia et al., 2003) and immunomodulatory (Antony et al., 1999) activities and are also screened for arsenic mitigation effects (Karim et al., 2010; Hazarika et al., 2015).

    • Sanguinarine: An evaluation of in vivo cytogenetic activity

      2004, Mutation Research - Genetic Toxicology and Environmental Mutagenesis
    View all citing articles on Scopus
    View full text