Factors affecting shoot proliferation and root initiation in organ cultures of the apple ‘Northern Spy’

doi:10.1016/S0304-4238(84)80006-0

Scientia Horticulturae

Volume 22, Issue 4, March 1984, Pages 347-358

https://doi.org/10.1016/S0304-4238(84)80006-0 Get rights and content

Abstract

Factors affecting the establishment, proliferation and adventitious root initiation of ‘Northern Spy’ are described. Shoot-tips established best in Linsmaier and Skoog medium with 5 μM BAP and 1 μM IBA supplemented with 1 mM phloroglucinol, but after the fourth sub-culture, proliferation was similar in media without phloroglucinol. Shoot proliferation was influenced by cytokinin type and concentration, light level and ex-plant type. Of the cytokinins tested, BAP was superior to zeatin, kinetin and 2iP. Proliferation was better with 10 μM BAP than with 5 or 1 μM BAP, but at 10 μM shoots were dwarfed. Single nodes and basal mass expiants produced significantly more shoots than did shoot-tips. Shoot proliferation was maximum at photon flux densities of 75 μmolm⁻²s⁻¹. Roots can be readily induced with IBA, but salt concentration and the physical support on which shoot-tips are grown influence both root initiation and growth. Half-strength salts and 1 μM IBA gave best root initiation. With agar, coarse sand, perlite and rotated liquid medium, 90–100% root initiation occurs, but root growth was poor in agar.

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Abstract

References (23)

Organic growth factor requirements of tobacco tissue cultures

Physiol. Plant.

Growth in vitro of isolated apple-shoot tips from young seedlings on media containing growth regulators

Bull. Acad. Polon. Sci., Cl. V

Embryogenesis in Citrus tissue culture

In vitro propagation of grapevine (Vitis vinifera L.) from fragmented shoot apices

Vitis.

Rapid in vitro rooting of the apple rootstock M.9

J. Hortic. Sci.

Report of East Mailing Research Station for 1979

Shoot and root initiation in vitro in the apple root-stock M.9 and the promotive effects of phloroglucinol

J. Hortic. Sci.

Effect of benzyl adenine on isolated apple shoots

Nature (London)

Effect of phloridzin and phloroglucinol on apple shoots

Nature (London)

Effect of phloridzin and phloroglucinol on apple shoots

Nature (London)

Propagation in vitro of M.26 apple rootstocks

J. Hortic. Sci.

Regeneration of apple shoots from shoot meristem-tips

Plant Sci. Lett.

Cited by (35)

Micropropagation of apple - A review

In vitro clonal propagation of an adult tree of neem (Azadirachta indica A. Juss.) by forced axillary branching

Protein variation and efficient in vitro culture of scale segments from Hyacinthus orientalis L. cv. Carnegie

Micropropagtion of apple cv. Tydeman's Early Worcester

Transformation of Apple Rootstock M26 with Agrobacterium tumefaciens

Establishment of cost-effective rooting, acclimatization and genetic fidelity of in vitro plants of apple rootstock Merton 793