Projections of pelvic autonomic neurons within the lower bowel of the male rat: an anterograde labelling study
Section snippets
Injection of DiI into the major pelvic ganglia in vivo
To gain an overview of the MPG projections to the gut via both the RN and PenN, DiI was injected into the MPG of 12 Advit male Wistar rats (106–245 g). Rats were anaesthetized with sodium pentobarbitone (48 mg/kg, i.p.), and the MPG were injected bilaterally with approximately 100–200 nl of DiI (diluted to 3–5% in ethanol) via a glass micropipette connected to a glass syringe filled with silicon oil, as described previously.[46]Two types of DiI were used [DiI(C12)3, n=9; DiI(C18)3, n=3; see
General features of DiI labelling
All fibres labelled anterogradely from injection of DiI into the MPG or from application of DiI onto the RN or PenN were confined to the middle and distal colon and rectum, with the majority of labelled structures occurring in the rectum. The primary target of MPG fibres within the gut was the mpx. A pelvic innervation of the serosal ganglia, and the circular muscle was also observed. Projections to the spx were rare and limited (1/9 animals had sparse spx labelling). An innervation of the
Discussion
These studies have provided the first description of the tissues targeted by rat MPG neurons in the lower bowel. They show that the majority of MPG projections innervate the mpx, although some supply the serosal ganglia and circular muscle. No other gut tissues are innervated by pelvic neurons. These results were produced by both in vivo and in vitro anterograde labelling of pelvic axons. The innervation of the mpx was primarily confined to the distal colon and rectum, with only a small
Conclusions
The present study has demonstrated that the major pelvic ganglia provide their primary innervation of the intestine to the myenteric plexus and are almost exclusively involved in the control of motility. These findings differentiate pelvic from prevertebral ganglia in two ways. First, unlike the pelvic ganglia, the prevertebral ganglia control secretory function and blood flow as well as motility. Second, while both ganglia control motility, the role of the pelvic ganglia is different due to
Acknowledgements
We wish to thank Mandy Bauer and Mark Kepper for their assistance with animal handling and tissue preparation, and Dr S. Brookes for teaching us the organotypic culturing method. This work was supported by the National Health and Medical Research Council of Australia.
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