Isolation and Taxonomic Characterization of a Halotolerant, Facultatively Iron-reducing Bacterium

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Summary

The isolation of a halotolerant, Fe(III)-reducing, Gram-negative bacterium from surface sediments of the Sippewisset marsh in Woodshole (USA) will be described. Detailed molecular taxonomic studies such as comparative 16S rRNA sequence analysis and DNA-DNA hybridization experiments revealed that the newly isolated strain is closely related to the previously isolated strain BrY. Both strains belong to the DNA-DNA similarity group IV of Shewanella putrefaciens which has recently been described as Shewanella alga. Thus, the ability to reduce Fe(III) is no longer restricted to S. putrefaciens within the genus but is also found among strains of S. alga. A 16S rRNA targeted probe has been designed that enabled us to rapidly differentiate strains of S. alga from those of S. putrefaciens and other bacteria.

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