Do land utilization patterns affect methanotrophic communities in a Chinese upland red soil?
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2016, Ecotoxicology and Environmental SafetyCitation Excerpt :Increased food requirements place greater pressure on agrarian practices, where crop yields and areas must increase to meet demand (Singh et al., 2011). Agricultural practices (including crop type, irrigation, organic amendments and fertilizer use) affect CH4 production and consumption as these microbially-mediated processes are regulated by the prevailing soil physico-chemical properties of agrarian soils and sediments (Zheng et al., 2010). Nitrogen (N) fertilizers are widely recognized as one of the key factors affecting CH4 oxidation in agricultural soils (Kravchenko et al., 2002; Seghers et al., 2003).
Isolation of methanotrophic bacteria from termite gut
2015, Microbiological ResearchCitation Excerpt :A melting curve completed the reaction with a starting temperature of 60 °C, which was held for 20 s and raised in steps of 20 s to 95 °C over a period of 30 min, which was again held for 20 s. The samples, containing specific MOB primer pairs (A189: 5′-GGNGACTGGGACTTCTGG-3′ and mb661: 5′-CCGGMGCAACGTCYTTACC-3′), were composed as described for BAC338F/BAC805R and the reaction was performed as follows: 95 °C for 2 min, 40 cycles of 94 °C for 1 min, 56 °C for 1 min, and 83 °C for 20 s, finalized with a melting curve described above (Zheng et al. 2010). The fatty acid analysis was carried out according to Dedysh et al. (2007) by the Department of Applied Microbiology of the Justus Liebig University in Gießen, Germany.
Differential response of archaeal groups to land use change in an acidic red soil
2013, Science of the Total EnvironmentCitation Excerpt :The soil sampling site was located at the Taoyuan Experimental Station of Agro-ecosystem Observation (28° 55′ N, 111° 26′ E) of the Chinese Academy of Sciences (Hunan Province, China). Detailed information about the experiment site has been described previously (Ying et al., 2010; Zheng et al., 2010). In brief, this long-term experiment started from the year of 1995 on a slightly sloped field, and five different land use types were initially set up.
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