Utilization of shrimp shellfish waste as a substrate for solid-state cultivation of Aspergillus sp. S1-13: Evaluation of a culture based on chitinase formation which is necessary for chitin-assimilation

doi:10.1016/S1389-1723(02)80236-5

Journal of Bioscience and Bioengineering

Volume 93, Issue 6, 2002, Pages 550-556

https://doi.org/10.1016/S1389-1723(02)80236-5 Get rights and content

Abstract

The utilization of shrimp shellfish waste as a substrate for solid-state cultivation of a filamentous fungus, Aspergillus sp. S1-13, was investigated. The organism was selected from among 220 isolates based on the productivity of its chitinolytic enzyme (chitinase), which might reflect microbial growth. The enzyme was produced only when the organism was grown on medium containing the shellfish waste. The addition of 58–65% water (w/w) to the medium was effective in enhancing production, and a certain amount of enzyme was observed in media of higher water content (up to about 75%). The initial pH and nitrogen source (ammonium sulfate) of the solid-state medium also affected the amount of enzyme. The amount of enzyme increased 2-fold in an optimum solid-state medium: 5 g of shrimp shellfish waste and 3 ml of basal medium (pH 5) containing 0.1% (NH₄)₂SO₄ was inoculated with 4 ml of spore suspension; static cultivation at room temperature. The amount increased further (1.5-fold) when the cultivation was carried out at 37°C, with 1.85 units of the enzyme formed from 1 g of shrimp shellfish waste. An analysis by ion-exchange column chromatography suggested the presence of at least two colloidal chitin-hydrolyzing enzymes and one p-nitrophenyl β-d-N-acetylglucosaminide-hydrolyzing enzyme in an extract of the solid-state culture. The elution profile was similar to that obtained with a liquid culture filtrate.

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Utilization of shrimp shellfish waste as a substrate for solid-state cultivation of Aspergillus sp. S1-13: Evaluation of a culture based on chitinase formation which is necessary for chitin-assimilation

Abstract

Enzyme Microb. Technol.

Enzyme Microb. Technol.

Enzyme Microb. Technol.

Enzyme Microb. Technol.

FEMS Microbiol. Lett.

Process Biochem.

Process Biochem.

Biochem. Eng. J.

Enzyme Microbiol. Technol.

Gene

Bioconversion of shellfish chitin wastes: process conception and selection of microorganisms

J. Food Sci.

Bioconversion of shellfish chitin waste: waste pretreatment, enzyme production, process design, and economic analysis

J. Food Sci.

Lactic acid fermentation of scampi waste in rotating horizontal bioreactor for chitin recovery

Process Biochem.

Solid state fermentations

Biotechnol. Bioeng.

Hydrolase production by Aspergillus niger in solid-state cultivation

Eur. J. Appl. Microbiol. Biotechnol.

Effect of particle size on enzyme production in solid state fermentation

Biores. Technol.

Real-time monitoring of the accretion of Rhizopus oligosporus biomass during the solid substrate fermentation

World J. Microbiol. Biotechnol.

Production of pectinase by Aspergillus niger in solid state fermentation at high initial glucose concentrations

World J. Microbiol. Biotechnol.