Comptes Rendus
no. 4
Photonique moleculaire : matériaux, physique et composants/Molecular photonics: materials, physics and devices
Molecular engineering of NLO-phores for new NLO microscopies
[Nouvelles techniques d'imagerie multiphonique : vers une nouvelle génération de marqueurs moléculaires]
Mireille Blanchard-Desce1
1 Synthèse et electrosynthèse organiques (CNRS UMR 6510), Institut de chimie, Université de Rennes 1, Campus scientifique de Beaulieu, bâtiment 10A, 35042 Rennes, France
Comptes Rendus. Physique, Volume 3 (2002) no. 4, pp. 439-448.

Les nouvelles techniques d'imagerie multiphotonique telles que la microscopie de génération de deuxième harmonique (SHG) et ou de fluorescence induite par excitation à deux photons (TPEF) connaissent à l'heure actuelle un véritable essor, de part les nombreux multiples avantages qu'elles offrent pour l'imagerie du vivant. Le développement de ces techniques passe par l'ingénierie de marqueurs moléculaires adaptés. Cette approche est illustrée par le design de chromophores et fluorophores membranaires optimisés pour l'imagerie SHG et/ou TPEF des membranes cellulaires. Au-delà d'une imagerie structurale, de tels marqueurs ouvrent également la voie à une véritable imagerie fonctionnelle.

Novel microscopies based on nonlinear optical (NLO) phenomena such as two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) have gained overwhelming popularity in the biology community owing to the many advantages they provide in biological imaging. Examples of molecular engineering approaches toward NLO-probes specifically designed for SHG and/or TPEF imaging of lipid membranes and biological cells are given here, providing an illustration of their intriguing potential in the area of real-time, non-damaging imaging of biological structures. Optimized NLO-markers open new routes for improved monitoring and better understanding of fundamental dynamic processes.

Accepté le :
Publié le :
DOI : 10.1016/S1631-0705(02)01329-4
Keywords: NLO-phores, fluorophores, two-photon absorption, TPEF, SHG, molecular probes, cell membranes
Mot clés : chromophores, fluorophores, absorption biphotonique, TPEF, SHG, sondes moléculaires, membranes cellulaires
Mireille Blanchard-Desce 1

1 Synthèse et electrosynthèse organiques (CNRS UMR 6510), Institut de chimie, Université de Rennes 1, Campus scientifique de Beaulieu, bâtiment 10A, 35042 Rennes, France 
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Mireille Blanchard-Desce. Molecular engineering of NLO-phores for new NLO microscopies. Comptes Rendus. Physique, Volume 3 (2002) no. 4, pp. 439-448. doi : 10.1016/S1631-0705(02)01329-4. https://comptes-rendus.academie-sciences.fr/physique/articles/10.1016/S1631-0705(02)01329-4/

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