Short communicationEffect of rhizobia symbiosis on lignin levels and forage quality in alfalfa (Medicago sativa L.)
Graphical abstract
An effect of rhizobia symbiosis on lignin level and forage quality in alfalfa. Rhizobia symbiosis increases lignin content and decreases forage digestibility. An increase in lignin content in nodulated alfalfa was associated with an upregulation of transcript levels of several lignin biosynthesis genes such as PAL, C4H, C3H, HCT and CCR. The upregulation of PAL gene may be linked to the increase of Ca level in alfalfa after nodulation. In addition, rhizobia symbiosis could also increase the levels of ADF, NDF and hemicellulose, resulting in a reduction in IVTD of alfafla. Therefore, forage quality was decreased due to the increase of lignin and other cell wall components (ADF, NDF and hemicellulose) induced by rhizobia symbiosis.
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Acknowledgments
This work was supported by the Project of National Natural Science Foundation of China (31272490, 31572456), the major Project for Tibetan forage industry (Z2014C02N02), China Postdoctoral Science Foundation (2014M552496) and Technology system of Agricultural Industry in China (CARS-35). We acknowledge the South Dakota Agricultural Experiment Station at South Dakota State University for technical assistance and language editing.
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