Journal of Plastic, Reconstructive & Aesthetic Surgery
Effect of local, long-term delivery of platelet-derived growth factor (PDGF) on injected fat graft survival in severe combined immunodeficient (SCID) mice
Section snippets
Animals
All experiments were performed with the prior approval of the St. Vincent's Hospital, Melbourne, Animal Ethics Committee, under the National Health and Medical Research Council Guidelines (Australia). Severe combined immune deficient (SCID) mice weighing between 20 and 25 g were used for this experiment. The SCID mouse model allows for the study of transplanted human fat without the immunological sequelae of rejection.20
Fat graft and experimental design
Fat was harvested by excision from the abdomen of an otherwise healthy
Statistical analysis
Groups were compared using one and two way analysis of variance (ANOVA) on SPSS v12.0. In the two way ANOVA, presence of spheres and addition of PDGF-BB were treated as the two independent variables. Alpha was set at 0.05.
Gross observations
There was no gross indication of acute infection, inflammation, abscess formation, seroma, or necrosis of the graft itself or the adjacent host tissue in any animal. Macroscopic observation of the fat grafts in all animals revealed consolidation of the grafted tissue into a
Discussion
Despite technical improvement in the procurement, handling, and delivery of free fat grafts, continued dissatisfaction with their survival has led to a variety of pharmaceutical interventions to improve the integrity of grafted adipocytes.14 Treatments have included insulin, steroids, vitamin E, lidocaine, epinephrine, and selective beta1 blockade.15, 16, 30, 31, 32 Although these interventions have displayed disappointing clinical efficacy, the focus on the biological behaviour of the
Acknowledgements
Dr. Randall Craft was funded by the Mayo Foundation. The authors thank Sue McKay, Liliana Pepe, and Anna Defteros for their surgical assistance.
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