Reproductive biology
Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome

https://doi.org/10.1016/j.fertnstert.2007.03.098Get rights and content
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Objective

To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome.

Design

Experimental study.

Setting

Research laboratory.

Animal(s)

Four- to five-week-old F1 (C57BL/6 × CBA/Ca) female mice.

Intervention(s)

Analysis by time-of-flight mass spectrometry.

Main Outcome Measure(s)

Protein profiles of denuded mouse metaphase II (MII) oocytes.

Result(s)

Metaphase II oocytes exposed to 1.5 M PrOH for 20 minutes with or without seeding (i.e., equilibration, dehydration with or without seeding) exhibited significantly altered protein profiles in comparison to controls (i.e., 37°C for 20 minutes and room temperature for 20 min). In total, 11 protein-biomarkers displayed profiles of statistically significantly lower abundance, whereas 8 protein-biomarkers were observed to be statistically significantly up-regulated. Protein profiles of MII oocytes maintained at room temperature for 20 minutes were more similar to those that were incubated for 20 minutes at 37°C.

Conclusion(s)

Analysis of the mouse MII oocyte at different stages of the slow-freezing protocol revealed a negative impact on oocyte physiology after PrOH exposure. In contrast, temperature changes per se (during cooling to room temperature) have limited effect on the oocyte proteome.

Key Words

Oocyte
slow freezing
1,2-propanediol
proteome

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