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Description of an Atlantic salmon (Salmo salar L.) type II interleukin-1 receptor cDNA and analysis of interleukin-1 receptor expression in amoebic gill disease-affected fish

https://doi.org/10.1016/j.fsi.2012.03.005Get rights and content

Abstract

Previously, we showed that IL-1β transcription is induced in the gills of amoebic gill disease (AGD)-affected fish in an AGD lesion-restricted fashion. However, in this environment, there is very little evidence of inflammation on histopathological or transcriptional levels and we hypothesised that aberrant signalling may occur. As a first step in investigating this issue, we cloned and sequenced the Atlantic salmon IL-1 receptor type II (IL-1RII) mRNA, and then examined the expression of both the IL-1RI (IL-1 receptor-like protein) and II during Neoparamoeba perurans infection. In gill lesions from AGD-affected fish, a step-wise temporal increase in the relative expression of IL-1β coincided with a significant reduction in IL-1RI, whereas the IL-1RII mRNA remained unchanged. Down-regulation of IL-1RI could explain the paucity of inflammation in affected tissue, although simultaneous up-regulation of IL-1β-inducible transcripts indicated that this is not due to a complete blockage of the IL-1RI pathway. Rather, it appears that IL-1RI transcription is reduced and this rate limits the effects of chronic IL-1β over-expression.

Highlights

► An Atlantic salmon interleukin-1 receptor type II cDNA is described. ► In AGD-affected gills, IL-1β, IL-1RI and IL-1RII mRNA expression was assessed. ► Polarised IL-1β & IL-1RI expression occurs in AGD-affected tissue. ► Down-regulation of IL-1RI may be compensatory for chronic IL-1β over-expression.

Section snippets

Acknowledgements

The authors would like to thank M. Attard and Dr P. Crosbie for their assistance. This work formed part of a project of Aquafin CRC and received funds from the Australian Government's CRCs Program, the Fisheries R & D Corporation and other CRC participants.

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    Present address: Faculty of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. Tel.: +61 414718875; fax: +61 3 97312366.

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