Review ArticleNeurotrophin gene augmentation by electrotransfer to improve cochlear implant hearing outcomes
Section snippets
Inner ear gene delivery
The translational potential of gene therapy in the inner ear is developing considerable prominence within the burgeoning domain of hearing therapeutics. This is founded upon successes in pre-clinical models that have utilised directed manipulation of gene expression to investigate developmental and physiological processes of hearing and balance at the molecular level. Modalities used for expression of recombinant proteins include ballistics (‘gene gun’-based delivery of gold particles coated
Background to gene electrotransfer
There has been a frameshift in understanding of pulsed electric field-based DNA transfer in recent years, with studies showing that electroporation is a misnomer in that plasmid DNA is too large to cross into cells through transiently generated pores in the plasma membrane, with associated electrophoretic translocation of the negatively charged DNA. Rather, it is now recognised that unlike small RNA and DNA oligonucleotide molecules, and fluorescent molecules such as propidium iodide, that
Declaration of interest
The BaDGE® registered trademark is assigned to UNSW Sydney Knowledge Exchange though New South Innovations Pty Ltd, which is also the assignee for the BaDGE®-related intellectual property.
Funding
Supported by funding from the Australian Research Council (ARC), grants (ARC DP151014754, ARC LP0992098, ARC LP140101008), the Garnett Passe and Rodney Williams Memorial Foundation, and the National Health and Medical Research Council (NHMRC) grants APP1091646, APP1122055 and GNT1142910. The research was supported by collaborative research funding from Cochlear Ltd.
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